Addgene

We narrowed to 20,931 results for: Tes

Showing: 4921 - 4950 of 20931 results

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pcDNA6-MRPS6-VN172

Plasmid

#182374

Purpose

mVenus-based mito-riboBiFC vector

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrps6 (Mrps6 Mouse)

Use

Tags

VN172

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact mApple

Plasmid

#187687

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-neoCV

Plasmid

#73889

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

neoCV

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTT135

Plasmid

#207924

Purpose

Premade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMP108

Plasmid

#207928

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A

Plasmid

#163619

Purpose

mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-mMcl-1

Plasmid

#25717

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (Mcl1 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Tags

Expression

Mutation

Promoter

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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Cenp-B-targeted Aurora B FRET sensor

Plasmid

#45232

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CENP-B targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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nonphosphorylatable Mis12-targeted Aurora B FRET sensor

Plasmid

#45229

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

non phosphorylatable Mis12-targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

T-A mutation in substrate sequence

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-FKBP-TEVp

Plasmid

#178867

Purpose

Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-FKBP-TEVprotease(Δ220-242)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Use

Tags

Flag-tag

Expression

Mutation

C160S

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Use

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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EYFP-Sec23A

Plasmid

#66611

Purpose

YFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
3918-37

Plasmid

#176495

Purpose

pTRE-EGFP-T2A-mCherry-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

Tetracyline Responsive Element driving EGFP-T2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA (405)

Plasmid

#121417

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat K41A HA (P#37)

Plasmid

#14655

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

K41A

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pEF-hSEMA4C-Fc

Plasmid

#74449

Purpose

Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

Depositor

Roland Friedel

Article

Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

Insert

human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)

Use

Tags

human Fc tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAPV-DsRed2-FLAG-ER-5

Plasmid

#170622

Purpose

Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

APV-DsRed2-ER-5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+5'MT-wbp2nl

Plasmid

#172467

Purpose

expresses myc-tagged version of wild-type protein

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

SP6

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b

Plasmid

#118325

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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MT21-Ubc9

Plasmid

#17273

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

Ubc9 (Ube2i Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pEBG-Pak1 83-149 L107F

Plasmid

#12215

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Autoinhibitory domain (aa 83-149) with L107F muta…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pAT206/Sept7shRNA

Plasmid

#38299

Depositor

Matthew Krummel

Article

Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

Insert

Septin 7 (Septin7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-STAT1

Plasmid

#117160

Purpose

Target site ATATCCAGTTCCTTTAGGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRF-AmeR

Plasmid

#49356

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AmeR repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

D120F, R121Q, G123H, Y124T, E125G, M126F, W127YS

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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