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Showing: 6681 - 6720 of 10097 results

  7. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233046
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233046-plasmid-map-sequence-id-467426

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  8. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233045
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233045-plasmid-map-sequence-id-467425

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  9. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  10. pDual_dsCas9_Puro_hs_TP73

    Plasmid
    #214689
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214689-plasmid-map-sequence-id-428791

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  11. pBABE 3xHA-MKK6-EE puro

    Plasmid
    #216791
    Purpose
    Mammalian expression of constitutively active human MKK6
    Depositor
    Kevin Janes
    Insert
    MKK6 (MAP2K6 Human)
    Use
    Retroviral
    Tags
    3x-HA
    Expression
    Mammalian
    Mutation
    S207E and T211E, constitutively active
    Promoter
    LTR
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216791-plasmid-map-sequence-id-434910

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  12. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  13. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  16. pTU1-A-yckABU

    Plasmid
    #112789
    Purpose
    Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    yckABU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112789-plasmid-map-sequence-id-218385

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  21. pAAV.CAG.iAChSnFR

    Plasmid
    #137955
    Purpose
    Expresses iAChSnFR under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Has Service
    AAV1 and AAV9
    Insert
    iAChSnFR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137955-plasmid-map-sequence-id-271572

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  25. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid
    #112677
    Purpose
    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Nuclear-localized floxed-mCherry EGFP
    Use
    AAV
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    112677-plasmid-map-sequence-id-237606

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  29. pAAV.hSynap.iAChSnFR

    Plasmid
    #137950
    Purpose
    Expresses iAChSnFR under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Has Service
    AAV1
    Insert
    iAChSnFR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynanpsin
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137950-plasmid-map-sequence-id-271681

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  30. pAAV.CAG.iAChSnFR-NULL

    Plasmid
    #137956
    Purpose
    Expresses iAChSnFR (non-binding variant) under CAG promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding variant)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Y140A binding site mutant
    Promoter
    CAG
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137956-plasmid-map-sequence-id-271715

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  35. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid
    #197500
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197500-plasmid-map-sequence-id-386637

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  38. pAAV.hSynap.iAChSnFR-NULL

    Plasmid
    #137951
    Purpose
    Expresses iAChSnFR (non-binding) under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (non-binding variant)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Y140A
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137951-plasmid-map-sequence-id-271588

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Showing: 6681 - 6720 of 10097 results