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We narrowed to 5,640 results for: pcas

Showing: 5041 - 5070 of 5640 results

  1. pHeCAHS-mEGFP-NLS

    Plasmid
    #205014
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205014-plasmid-map-sequence-id-405259

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  5. pR008_APAD

    Plasmid
    #204818
    Purpose
    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control
    Depositor
    Xiaochang Zhang
    Article
    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:
    Insert
    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    Use
    Tags
    HA and Flag and huADAR2dd
    Expression
    Mammalian
    Mutation
    Promoter
    chicken β-actin promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204818-plasmid-map-sequence-id-403437

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  6. AAEL007097-Cas9

    Plasmid
    #100705
    Purpose
    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Mutation
    Promoter
    AAEL007097 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100705-plasmid-map-sequence-id-193542

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  11. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197979
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197979-plasmid-map-sequence-id-388023

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  12. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  15. AAEL003877-Cas9

    Plasmid
    #100581
    Purpose
    The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL003877-hspcas9-GFP
    Opie-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Mutation
    Promoter
    AAEL003877 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100581-plasmid-map-sequence-id-194325

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  17. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197966
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CmYLCVp and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197966-plasmid-map-sequence-id-387890

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  18. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211784-plasmid-map-sequence-id-417361

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  19. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  22. PB-TAC-OKMS-Cas9

    Plasmid
    #120353
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120353-plasmid-map-sequence-id-236054

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  23. pX330 _HITI donor B6

    Plasmid
    #172847
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172847-plasmid-map-sequence-id-341030

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  25. pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197981
    Purpose
    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-nCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197981-plasmid-map-sequence-id-388026

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  27. pRvCAHS1-mEGFP-NLS

    Plasmid
    #205013
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205013-plasmid-map-sequence-id-405257

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  28. pRvSAHS1-mCherry-NLS

    Plasmid
    #205009
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205009-plasmid-map-sequence-id-405252

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Showing: 5041 - 5070 of 5640 results