We narrowed to 4,886 results for: phy

1. Human HMG-CoA lyase (HL)

   Plasmid

   #52433

   Purpose

   bacterial expression of human HMG-CoA lyase (HL)

   Depositor

   Henry Miziorko

   Article

   Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

   Insert

   HMG CoA lyase (HMGCL Human)
   

   Use

   Tags

   His and S

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBAD_His B_QuasAr1

   Plasmid

   #64135

   Purpose

   Fluorescent reporter for membrane voltage with improved brightness

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-CreLite

   Plasmid

   #131782

   Purpose

   Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox; Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFL-EGFP-3xFlag-Fml1

   Plasmid

   #193058

   Purpose

   Baculovirus expression of S.pombe Fml1 for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   Fml1 (fml1 Fission Yeast)
   

   Use

   Tags

   3xFlag

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFE-hCA

   Plasmid

   #132999

   Purpose

   pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   hCAII (CA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJC6-GPT1-3xFLAG

   Plasmid

   #163448

   Purpose

   lentiviral overexpression vector for human GPT1

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GPT1 (GPT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_FGFR2

   Plasmid

   #187751

   Purpose

   MAC-tagged gene expression of human FGFR2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   FGFR2 (FGFR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. attB-­GFP-­Poly(A)‐NeoR

   Plasmid

   #65526

   Purpose

   NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-human C-MYC-V394D

   Plasmid

   #74162

   Purpose

   Mammalian expression of human cMyc VD mutant

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V394D

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-51b(+)-Caprola_14

    Plasmid

    #194675

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_14
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGS1034

    Plasmid

    #63796

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

    Insert

    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    

    Use

    E. coli cloning vector

    Tags

    CBP-3xFLAG-TEV-Protein A

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVneo-HOXB3-FLAG

    Plasmid

    #74508

    Purpose

    Retroviral expression of HOXB3

    Depositor

    Lars Rönnstrand

    Article

    Lindblad et al Biochem Biophys Res Commun. 2015 Nov 27;

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. MAC_C_ERBB2

    Plasmid

    #187776

    Purpose

    MAC-tagged gene expression of human ERBB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. EGFR∆550-580-mEGFP

    Plasmid

    #203788

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    aa 550-580 deleted

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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