We narrowed to 1,583 results for: pbluescript
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Plasmid#173686PurposeThe expression vector for genes of interest under mouse calmegin promoter.DepositorTypeEmpty backboneExpressionMammalianPromotermouse Clgn promoterAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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SpyCas9 EMX1-nicking sgRNA
Plasmid#169859PurposeSpyCas9 nicking sgRNA for EMX1DepositorInsertSpyCas9 EMX1-nicking sgRNA
ExpressionMammalianPromoterhuman U6 promoterAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
TU6m-gRNAscaffHygroR
Plasmid#165485PurposeS. pyogenes Cas9 guide RNA expression in tilapia cellsDepositorTypeEmpty backboneUseCRISPRPromoterOreochromis mossambicus U6Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL24a-Linker-smHA-FP-DHFR-LoxP
Plasmid#166238PurposeTagging of weakly expressed genes, floxed DHFR markerDepositorInsertSpaghetti monster HA
MutationnoneAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone
Plasmid#164266PurposesgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.DepositorTypeEmpty backboneUseCRISPRExpressionWormPromoterU6 promoter from W05B2.8Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-mEGFP
Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-mEGFP
Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-mEGFP
Plasmid#115910Purposedonor to insert EGFP at the SEC61B locus in human cellsDepositorAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-dTomato
Plasmid#115919Purposedonor to insert IRES-dTomato at the GAPDH locus in human cellsDepositorInsertIRES-dTomato
UseDonor templateTagsdTomatoAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVIPR2-ERT2CreERT2pA
Plasmid#163628PurposeHeart-specific tamoxifen-dependent Cre expressionDepositorInsertCre recombinase
UseCre/LoxTagsERT2 on both N-terminal and C-terminal of CreExpressionMammalianAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB Neo* attP
Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31ExpressionBacterial and MammalianAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCre-AntiDsRed
Plasmid#139674PurposeCassette for testing Cre recombinase activity using a DsRed2 fluorescent reporterDepositorInsertAntiDsRed Cre recombinase
ExpressionPlantPromoterMaize UbiquitinAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTBxb1
Plasmid#127536PurposePlasmid encodes A. thaliana codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS2
Plasmid#139410PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.DepositorInsertGFP S65T
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBORF-DBD
Plasmid#127463PurposeORF filtering plasmid (lambda cI associated plasmid for pAVA)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSDMA66
Plasmid#128355PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorInsertNoureothricin (NAT)
UseUnspecifiedPromoterACT1Available SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
sCFP in pBSDONR P4r-P2
Plasmid#121530PurposeFor making C-terminal sCFP fusions using multisite Gateway cloningDepositorInsertsCFP
ExpressionBacterialAvailable SinceAug. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bluescript-OPNb
Plasmid#107979Purposeexpression of OPNbDepositorInsertosteopontin (OPNb)
ExpressionBacterialAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK409 (mAID-Nluc BSD)
Plasmid#121199PurposeC-terminal taggingDepositorInsertmAID-Nluc BSD
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK388 (mClover-BSD)
Plasmid#121189PurposeC-terminal taggingDepositorInsertmClover-BSD
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
b2AR->M71-IRES-tauGFP-ACNF TV
Plasmid#105198Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.DepositorAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Trpc2-IRES-taumCherry-ACNF TV
Plasmid#105212Purposetargeting vector to generate a Trpc2-IRES-taumCherry mouse strain.DepositorInsertTrpc2-IRES-taumCherry-ACNF (Trpc2 Mouse)
UseMouse TargetingAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-IRES-tauGFP-ACNF TV
Plasmid#105211Purposetargeting vector to generate a Gucy1b2-IRES-tauGFP mouse strain.DepositorInsertGucy1b2-IRES-tauGFP-ACNF (Gucy1b2 Mouse)
UseMouse TargetingAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3-HPRT1-S104R-PdTK-mCh
Plasmid#107276PurposeHPRT1 c.312C>A Munich donor vector with unilateral microhomologyDepositorInsertsMutationc.306G>T, c.312C>AAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
ExpressionBacterialAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TTN/pBSSK+
Plasmid#98124Purposetitin (NM_133378, nt 99776 to 100510) in pBSSK+DepositorInsertTTN (TTN Human)
UseUnspecifiedAvailable SinceAug. 18, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-AMVpl-β1WT-nAchR
Plasmid#97080PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertBeta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
366 pTol2-CherryDest
Plasmid#73487Purposepromoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertmCherry-attR1/attR2
MutationH30R (please see depositor comments below)Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-MKOS
Plasmid#80484PurposepiggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianPromotertetOAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
acta 2
Plasmid#78711Purposezebrafish acta2 3-UTR–specific probeDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS B2rescue A70D
Plasmid#78658PurposeGenerates B2 inserts suitable for rescueDepositorInsertB2rescue cassette
ExpressionMammalianAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJR50
Plasmid#75026PurposeU6 promoter driven flipped + extended sgRNA expression vectorDepositorInsertguide RNA, flipped and extended version
UseCRISPRPromoterR07E5.16 (U6)Available SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
370 pTol2-lmo2:CherryDest
Plasmid#73489PurposeTol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signalDepositorInsertlmo2 promoter:mCherry-attR1-attR2
Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-TetR-cryaa-YFP
Plasmid#73202PurposeBAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker CitrineDepositorInsertYFP
Available SinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
369 pTol-lmo2:egfpDest
Plasmid#73488PurposeTol2 vector with lmo2 promoter upstream of EGFP, attR1/R2 cassette and late SV40 pA signalDepositorInsertlmo2 promoter:EGFP-attR1-attR2
Available SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi gamma-tubulin shRNA mouse
Plasmid#70666PurposeReduced the expression of gamma-tubulin in murine cellsDepositorInsertUseU6 promoterExpressionMammalianPromoterU6Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8WTmneo
Plasmid#69609PurposeExpresses a neomycin tagged wildtype L1PA8DepositorInsertL1PA8
Tagsmneo retrotransposition cassetteExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gfap-Cryab
Plasmid#58947Purposehamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytesDepositorInsertCryab
UseMouse Targeting; Transgene expressionExpressionMammalianMutationgenomic fragmentPromoterhuman GFAP promoter (position -2163 to +47)Available SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDY4
Plasmid#53589PurposeProduces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.DepositorInsertlcyE
ExpressionBacterialPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_3_XcmI-LacZ
Plasmid#49298PurposeGolden Gate entry vector at position 3, LacZDepositorTypeEmpty backboneUseCloning vectorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_+nls-eGFP-HA-_+1_BK+
Plasmid#49312PurposeGolden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codonDepositorInsertnls-EGFP-HA
UseCloning vectorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT366_pattB-Hb9-GFP-pA-FRT5
Plasmid#52552Purposefor PhiC31 integrase-mediated integration of pHb9-GFPDepositorInsertsPhiC31o attB (full length)
GFP
ExpressionMammalianAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
Plasmid#52540PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
ExpressionMammalianAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialPromoterPtrc-T7Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNtCp-rbcL-PHLS-GPPS-aadA-accD
Plasmid#52471PurposeInserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacumDepositorInsertrbcL-PHLS-GPPS-aadA-accD
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNtCp-rbcL-PHLS-aadA-accD
Plasmid#52470PurposeInserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacumDepositorInsertrbcL-PHLS-aadA-accD
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNtCp-rbcL-accD
Plasmid#52469PurposeContains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformationDepositorInsertrbcL-accd Nicotiana tabacum Cp DNA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2-PHLS (b)
Plasmid#52307PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialPromoterpsbA2Available SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-PHLS (d)
Plasmid#52309PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialPromoterPtrcAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only