We narrowed to 5,122 results for: NOG

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV3FLAG_hGTPBP4

Plasmid

#53974

Purpose

Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

GTPBP4 (GTPBP4 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_VHH-GFP4::CD8::mCherry

Plasmid

#163917

Purpose

Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

Depositor

Markus Affolter

Article

Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

Insert

Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(A)

Plasmid

#87913

Purpose

Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499A

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

Plasmid

#160611

Purpose

Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:LbCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Ras85D CA

Plasmid

#52247

Purpose

Drosophila expression of YFP-Ras85D constitutively active mutant

Depositor

Matthew Scott

Insert

Ras85D (Ras85D Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-BECN1

Plasmid

#99328

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

Beclin 1 (BECN1 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Insect

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

Plasmid

#163928

Purpose

Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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908H

Plasmid

#160240

Purpose

TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00532

Plasmid

#39630

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3629 (Dll Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pJJGL002

Plasmid

#180606

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9-VRER_2A_GFP

Plasmid

#75475

Purpose

Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

Depositor

Marc Tessier-Lavigne

Article

Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

Insert

Cas9-VRER

Use

CRISPR

Tags

2A_GFP

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

CAG

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Mutation

Mutated amino acids in catalytic triad to alanine…

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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