59,508 results

1. pTCF158 Human beta-2 microglobulin

   Plasmid

   #180457

   Purpose

   E. coli expression of class I MHC light chain

   Depositor

   John Altman

   Insert

   Human beta-2 microglobulin
   

   Expression

   Bacterial

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCB-hDot1Lwt

   Plasmid

   #74173

   Purpose

   For packaging hDot1Lwt to retrovirus.

   Depositor

   Yi Zhang

   Article

   Okada et al Cell. 2005 Apr 22;121(2):167-78.

   Insert

   hDot1L (DOT1L Human)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. BcatMutS33/S37.T41/S45

   Plasmid

   #24204

   Depositor

   David Rimm

   Insert

   beta catenin (CTNNB1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S33A, S37A, T41A,S45A

   Promoter

   CMV

   Available Since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-p18-HA

   Plasmid

   #42338

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 (LAMTOR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. LEGO-pDHS-ITDE-AP1x6-DUSP5

   Plasmid

   #217419

   Purpose

   Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

   Depositor

   Peter Cockerill

   Insert

   Luciferase, GFP
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Human DUSP5 promoter

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

   Plasmid

   #164611

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Expression

   Mammalian

   Mutation

   S383T

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-LMNB1

   Plasmid

   #207770

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-off TauRD (P301L/V337M)

   Plasmid

   #188572

   Purpose

   Dox-repressible expression of the Tau repeat domain

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   Tau repeat domain (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. sFlt1-i13 V5

    Plasmid

    #86044

    Purpose

    human Soluble Flt1 isoform i13 (terminates in intron 13)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform i13 (FLT1 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform i13 (terminates in intron 13)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217342

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3164

    Plasmid

    #144640

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGKp-GFP-YAP (5SA)

    Plasmid

    #174174

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Hs.HRAS G12V

    Plasmid

    #83184

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12V

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc-GFPC1-Sec61beta

    Plasmid

    #15108

    Depositor

    Tom Rapoport

    Insert

    Sec61beta (SEC61B Human)
    

    Tags

    Ac GFP

    Expression

    Mammalian

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. NES-EGFP-PH-ARNO2G-I303Ex2

    Plasmid

    #116868

    Purpose

    PIP3 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    CYTH2 (CYTH2 Human)
    

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amind acids 252-399, isoleucine 303 changed to gl…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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