Addgene

We narrowed to 8,068 results for: gnal

Showing: 5101 - 5130 of 8068 results

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3xNLS-NLP-cMyc-cMyc LbaCas12a

Plasmid

#182125

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Plasmid

#182124

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1

Plasmid

#187010

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (S129D)

Plasmid

#185719

Purpose

AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ser 129 to Asp

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (S129A)

Plasmid

#185718

Purpose

AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ser 129 to Ala

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-mSNCA(1-95)

Plasmid

#185716

Purpose

AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (Snca Mouse)

Use

AAV

Tags

Expression

Mutation

deleted AA 95-144

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GFP11-RhoA

Plasmid

#181860

Purpose

Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-RhoA (RHOA Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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ETS2_pLENTI-CAG-IRES-GFP

Plasmid

#177005

Purpose

Mammalian lentiviral expression vector encoding ETS2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ETS2 (ETS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MOSMO_pcDNA6.2/EmGFP-Bsd

Plasmid

#176975

Purpose

Mammalian expression vector encoding MOSMO and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MOSMO (C16orf52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CYYR1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176986

Purpose

Mammalian expression vector encoding CYYR1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CYYR1 (CYYR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CBR3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176961

Purpose

Mammalian expression vector encoding CBR3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CBR3 (CBR3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CLDN8_pcDNA6.2/EmGFP-Bsd

Plasmid

#176963

Purpose

Mammalian expression vector encoding CLDN8 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CLDN8 (CLDN8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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FAM207A_pcDNA6.2/EmGFP-Bsd

Plasmid

#176950

Purpose

Mammalian expression vector encoding FAM207A and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

FAM207A (C21orf70 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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PX459_GRHL1-exon5

Plasmid

#177763

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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PX459_GRHL1-exon8

Plasmid

#177764

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

Plasmid

#177767

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

R9A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

Plasmid

#177768

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S95A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

Plasmid

#177769

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K116A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

Plasmid

#177770

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

T139A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K370A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

Plasmid

#177772

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S374A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K592A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Use

Tags

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

Plasmid

#175256

Purpose

SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ST-Tstem-GFP

Plasmid

#162501

Purpose

Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The Tac stem region is inserted within ST6Gal1

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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