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pcDNA3puro-mGFP-FXR1-isoform a
Plasmid#225447PurposeExpress EGFP-fusion protein of FXR1 isoform aDepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE PGK-GFP-IRES-LUC-W
Plasmid#46793Purpose2nd generation lentiviral vectorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUGW-ER-TurboID
Plasmid#200965Purpose3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFPDepositorInsertER-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CMV-GFP-BGHpA
Plasmid#190239PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pQCXIP GFP-MOSPD2 WT
Plasmid#186467PurposeExpression of human MOSPD2 fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP
Plasmid#196988PurposeDerived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vectorDepositorInsertNestin-dCas9-KRAB-T2a-GFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-CIBN-GFP
Plasmid#79572Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFPDepositorInsertLyn11
UseTagsCIBN (CRY2-binding domain) and EGFPExpressionMammalianMutationG8R (please see depositor comments below)PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA-mGFP
Plasmid#180791PurposeExpression of human BTLA fused to mEGFPDepositorInsertBTLA (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSD080A
Plasmid#218138PurposeLentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.DepositorInsertSD40-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSF1-GFPN3
Plasmid#32538DepositorInsertHSF1 (HSF1 Human)
UseTagsEGFPExpressionMammalianMutationsee depositor comments belowPromoterCMVAvailable sinceOct. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 67kDa
Plasmid#201361PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 67kDa
Plasmid#201372PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-WT
Plasmid#133751PurposeWild-type Numb tagged with GFP cloned into PWPXLDDepositorInsertGFP-Numb (Numb Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid
Plasmid#169732PurposeEqualizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xPCP-2xGFP
Plasmid#71243PurposeUsed to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.DepositorInsert2xPCP-2xGFP
UseP element-mediated germline transformationTagsEGFPExpressionInsectMutationPromoterHsp83Available sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-C(3+)-EiP
Plasmid#21313PurposeLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-C(3+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-S(4+)-EiP
Plasmid#21314PurposeLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-S(4+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Golgi-TurboID
Plasmid#200964Purpose3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFPDepositorInsertGolgi-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-H plasmid
Plasmid#169367PurposeEqualizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF3) target-miR(FF3)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-M plasmid
Plasmid#169731PurposeEqualizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 41kDa
Plasmid#201342PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant
Plasmid#225462PurposeExpress mEGFP-fusion protein of FMR1 isoform 1DepositorInsertFMR1 isoform 1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pHR-PD-1 G224A-mGFP
Plasmid#180819PurposeExpression of human PD-1 G224A fused to mEGFPDepositorInsertPD-1 (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationG224APromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyFlex
Plasmid#218234PurposeA piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIpA102-G-HLA-A2_GFP
Plasmid#85162Purposefor mRNA expression of HLA.A2-EGFPDepositorInsertHLA.A2 (HLA-A Human)
UseMrna expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSD141B
Plasmid#218139PurposeLentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.DepositorInsertSD56-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha pcp gfp
Plasmid#64539PurposeUsed to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.DepositorInsertPCP
UseLentiviralTagsEGFP, FactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Diffusive 41kDa
Plasmid#201335PurposeNucleocytoplasmic transport probeDepositorInsertEGFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-mouse vinculin full length (889)
Plasmid#67935PurposeMammalian expression of mouse vinculin with GFP fusionDepositorInsertVinculin (Vcl Mouse)
UseTagsEGFPExpressionMammalianMutationL159P in vinculinPromoterCMVAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2_UBE2:eG-Lt-CsA
Plasmid#136134PurposePlasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:eGFP-Lti6b
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70
Plasmid#62951PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
IRES-GFP-AXL-KD
Plasmid#65498PurposeExpresses kinase dead AXL in mammalian cells with cistronic EGFPDepositorInsertTyrosine-protein kinase receptor UFO (AXL Human)
UseTagsFLAGExpressionMammalianMutationK567RPromoterCMVAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reduced Expression GFP beta actin
Plasmid#31502DepositorInsertbeta actin (ACTB Human)
UseTagsEGFPExpressionMammalianMutation91-544 of enhancer region of CMV promoter deletedPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1(R40L)-GFP
Plasmid#51408PurposeNon-PI(4,5)P2 binding control for PH-PLCD1-GFPDepositorInsertPH-PLCD1 (aa 1–170) (PLCD1 Human)
UseTagsEGFPExpressionMammalianMutationArginine 40 mutated to leucinePromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTfR-PATagRFP
Plasmid#31946DepositorInsertPATagRFP
UseTagsTfRExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEM047 [T7-assisted CROPseq sgRNA expression vector]
Plasmid#224899PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNADepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only
