We narrowed to 13,780 results for: TIM

1. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

   Plasmid

   #108960

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-zfCSF3b

   Plasmid

   #65613

   Purpose

   express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

4. LT3REVIN

   Plasmid

   #111175

   Purpose

   Tet-regulated miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   T3G

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 shTGFBR3 puro

   Plasmid

   #58696

   Purpose

   Lentiviral shRNA vector for knockdown of human TGFBR3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   shTGFBR3 (TGFBR3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pSR02

   Plasmid

   #69149

   Purpose

   Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   rps-27

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRZ-mNanog-YNL

   Plasmid

   #65717

   Purpose

   mNanog reporter plasmid (expresses YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   murine Nanog promoter and YNL
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPMVAd36

   Plasmid

   #98302

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5 Flag-Smad2 EPSM

   Plasmid

   #14933

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

   Insert

   Smad2 EPSM (SMAD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Erk/Pro-directed kinase site mutant (EPSM). T220V…

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pJH4687

    Plasmid

    #200783

    Purpose

    Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    ins-22 (ins-22 Nematode)
    

    Use

    Tags

    pHiuorin

    Expression

    Worm

    Mutation

    Promoter

    Pnpr-4

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. CytERM-mTurquoise2

    Plasmid

    #98833

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    CytERM
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXNgate21-3HA

    Plasmid

    #105078

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pROF394

    Plasmid

    #155333

    Purpose

    Expression of PULSE-controlled LFY-VP16-2A-RLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RT3GEP

    Plasmid

    #111164

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TetR-NLS-VP16

    Plasmid

    #103834

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GADD45 P-2/3m-luc

    Plasmid

    #8359

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter P-2/3m (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    P-2/3m

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pIND-CLucZ

    Plasmid

    #53224

    Purpose

    Inducible expression of Cypridina luciferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Cypridina luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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