We narrowed to 5,300 results for: pcDNA3.1+

1. pcDNA-Flag-Kdm6b(1025-End)-pA

   Plasmid

   #100278

   Purpose

   In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

   Insert

   Kdm6b (Kdm6b Mouse)
   

   Use

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   catalytic domain (1025aa-End)

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-NHERF1

   Plasmid

   #31650

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
   

   Use

   Tags

   His & S tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-GFP-BIRC3-NU

   Plasmid

   #160698

   Purpose

   Expresses GFP-BIRC3 without the endogenous 3'UTR in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP-BIRC3 (BIRC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLSx3-CRY2PHR-p65

   Plasmid

   #64124

   Purpose

   Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   CRY2PHR-p65
   

   Use

   Tags

   His, NLSx3, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIR-FbGFPY58/C-PKI

   Plasmid

   #184685

   Purpose

   NIR-Fb-based PKA inhibitor

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFPY58/C-PKI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. human-GFAP-EGFP

   Plasmid

   #169473

   Purpose

   We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. BFP Homology Donor

   Plasmid

   #149372

   Purpose

   Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Insert

   CDDR BFP Homology Donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK

   Plasmid

   #62670

   Purpose

   Vector for PhiC31o mRNA synthesis

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-miRFP718nano

   Plasmid

   #195744

   Purpose

   Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mH2A1.2-CT-GFP

    Plasmid

    #45169

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-2xCL-YFP-Vpr

    Plasmid

    #105215

    Purpose

    Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

    Depositor

    Gregory Melikyan

    Article

    Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

    Insert

    Vpr
    

    Use

    Tags

    PC2x (SQNY/IRKVL), eYFP, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. perbB2-ECFP

    Plasmid

    #40268

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    ecfp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHLuc Control

    Plasmid

    #141091

    Purpose

    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mc2 mNeon EF1a

    Plasmid

    #206224

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 CHICA (Nigg HS342)

    Plasmid

    #41153

    Depositor

    Erich Nigg

    Article

    Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

    Insert

    CHICA (FAM83D Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    V11L

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH2.19_mCherry-MS2x2

    Plasmid

    #205534

    Purpose

    Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG SMG1 D2335A/N2340K

    Plasmid

    #199575

    Purpose

    Expresses FLAG-tagged kinase dead SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D2335A and N2340K

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmyc-GFP-TNRC6B

    Plasmid

    #42022

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6B (TNRC6B Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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