We narrowed to 23,602 results for: CHI

1. pDONR P4-P1R-V5-6xHis

   Plasmid

   #48346

   Purpose

   Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   V5 and 6xHis epitope tags cassette
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Contains a Kozak sequence and initiation codon up…

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. NET37 pmRFP-N2 (450)

   Plasmid

   #61980

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET37 (KIAA1161 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMADM-alpha

   Plasmid

   #36890

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   Neo
   ATG (T)
   GFP
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, deleted…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT259_(pRosa26-G-tTA2)

   Plasmid

   #36878

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   diphteria toxin A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-α-COPI-WD40

   Plasmid

   #180758

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Leu181Lys, Leu…

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20 CARD8-HA PAM S297A

   Plasmid

   #169983

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, S297A

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. cmv hTid Long H121Q

   Plasmid

   #13708

   Depositor

   Karl Munger

   Article

   Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

   Insert

   hTid Long H121Q (DNAJA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H121Q

   Promoter

   Available since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET22b-ecDHFR-N23PP/G51PEKN

   Plasmid

   #118583

   Purpose

   Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, G51 mutated to P51, a Pro res…

   Promoter

   T7

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 NLRP1 M77T

    Plasmid

    #166818

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    M77T

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 NLRP1 A54T

    Plasmid

    #166815

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A54T

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

    Plasmid

    #166832

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-hPMCA2x/b delta6

    Plasmid

    #47587

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2x/b delta6 (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pRK5R DD-FAM98B[1-330]

    Plasmid

    #211358

    Purpose

    Transient expression of DD-V5-FAM98B[1-330]

    Depositor

    Raghu Chivukula

    Article

    Yang et al Science. 2025 Jul 17;389(6757):eado2403.

    Insert

    DD-FAM98B[1-330] (FAM98B Human)
    

    Use

    Tags

    DD-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-SOS1(dog)-gRNA1

    Plasmid

    #228755

    Purpose

    A knockout vector for dog SOS1.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    A gRNA targeting the dog SOS1 gene and the cDNA of CRISPR-Cas9 (SOS1 canis lupus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-SOS1(dog)-gRNA2

    Plasmid

    #228756

    Purpose

    A knockout vector for dog SOS1.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    A gRNA targeting the dog SOS1 gene and the cDNA of CRISPR-Cas9 (SOS1 canis lupus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57-CMV-MAAP6

    Plasmid

    #218245

    Purpose

    1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW57-CMV-MAAP9

    Plasmid

    #218246

    Purpose

    1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

    Plasmid

    #216171

    Purpose

    Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Expression

    Mutation

    Promoter

    EF1a, hU6, and mU6

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-Myc-FOXA3

    Plasmid

    #219395

    Purpose

    Express FOXA3 in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid

    #213417

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid

    #213414

    Purpose

    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A-E1021A (VCL Chicken, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pME map2k2a_s219D

    Plasmid

    #201058

    Purpose

    Gateway middle entry vector expressing constitutively active zebrafish map2k2a

    Depositor

    Sarah Childs

    Article

    Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

    Insert

    MAP2k2a (map2k2a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Mutation S219D, constitutively active

    Promoter

    none

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLINE1mORF1-ΔORF2-scFvFc

    Plasmid

    #206023

    Purpose

    The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. psicheck2-PHLPP2mut

    Plasmid

    #203606

    Purpose

    Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    miR-27b target sequences in the 3'UTR were m…

    Promoter

    SV40

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CSII-TK-B4GALT3

    Plasmid

    #203598

    Purpose

    Lentiviral vector to express B4GALT3 in mammalian cells

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TK

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CSII-TK-MAIP1

    Plasmid

    #203599

    Purpose

    Lentiviral vector to express MAIP1 in mammalian cells

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    matrix AAA peptidase interacting protein 1 (MAIP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TK

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. psicheck2-B4GALT3

    Plasmid

    #203601

    Purpose

    Reporter plasmid carrying B4GALT3 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    nt 1800-2330 3'UTR

    Promoter

    SV40

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. psicheck2-MAIP1

    Plasmid

    #203603

    Purpose

    Reporter plasmid carrying MAIP1 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    matrix AAA peptidase interacting protein 1 (MAIP1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge