We narrowed to 9,406 results for: Coli

1. pKG326

   Plasmid

   #69753

   Purpose

   Retroviral expression of CENP-T aa 1-531

   Depositor

   Iain Cheeseman

   Insert

   CENP-T aa 1-531 (CENPT Human)
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Mutation

   E.coli optimized sequence; A216T and G459R mutati…

   Promoter

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pJKW0825

   Plasmid

   #119022

   Purpose

   E.coli expression plasmid for Pm4CL1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   Pm4CL1
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJKW1574

   Plasmid

   #119178

   Purpose

   E.coli expression plasmid for Pm4CL1 and PmSPS1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Inserts

   Pm4CL1
   PmSPS1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pGAP

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RNb-zdk1

   Plasmid

   #128781

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   RNb-zdk1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJKW1659

   Plasmid

   #119180

   Purpose

   E.coli expression plasmid for Pm4CL1 and PmCHS

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Inserts

   Pm4CL1
   PmCHS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pGAP

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAl-pelB-DB21

   Plasmid

   #226994

   Purpose

   Express nanobody DB21 in E.coli

   Depositor

   Ning Zheng

   Article

   Cao et al Nat Commun. 2022 Feb 10;13(1):815. doi:

   Insert

   DB21
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAl-pelB-CA14

   Plasmid

   #226993

   Purpose

   Express nanobody CA14 in E.coli

   Depositor

   Ning Zheng

   Article

   Cao et al Nat Commun. 2022 Feb 10;13(1):815. doi:

   Insert

   CA14
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RNb-SEpHluorin

   Plasmid

   #128785

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   RNb-SEpHluorin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ssRNb-GFP

   Plasmid

   #128791

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   ssRNb-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJKW1077

    Plasmid

    #119025

    Purpose

    E.coli expression plasmid for PmCHS

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Insert

    PmCHS
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBUN82

    Plasmid

    #60675

    Purpose

    overexpression of M. smegmatis alrA gene in Mycobacteria

    Depositor

    Raul Barletta

    Article

    Caceres et al J Bacteriol. 1997 Aug;179(16):5046-55.

    Insert

    alrA
    

    Use

    E.coli-mycobacterium shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    hsp60

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET22b-EC12-129/138Avi

    Plasmid

    #47927

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi tag in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis, Avi Tag, and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR

    Plasmid

    #42875

    Purpose

    A crRNA expression plasmid for targeting a specific sequence.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR-BsaI
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCB-SC101-Spe

    Plasmid

    #133765

    Purpose

    shuttle vector backbone

    Depositor

    Himadri Pakrasi

    Article

    Liu et al Microb Cell Fact. 2018 Mar 26;17(1):48.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector for e.coli and …

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBUN250

    Plasmid

    #60672

    Purpose

    overexpression of M. smegmatis ddl gene in Mycobacterium

    Depositor

    Raul Barletta

    Article

    Feng et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    ddl
    

    Use

    E.coli-mycobacterium shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    BCG Hsp60

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-6xHis-(pos16)GFP

    Plasmid

    #89249

    Purpose

    Expresses GFP with +16 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-PFO

    Plasmid

    #92109

    Purpose

    Recombinant expression in E.Coli

    Depositor

    Feng Shao

    Article

    Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

    Insert

    PFO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Delta27aa

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBXCA3H

    Plasmid

    #47072

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-6xHis-(pos9)GFP

    Plasmid

    #89247

    Purpose

    Expresses GFP with +9 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos9)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCoofy61

    Plasmid

    #122013

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

    Depositor

    Sabine Suppmann, Peggy Stolt-Bergner

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJKW1658

    Plasmid

    #119179

    Purpose

    E.coli expression plasmid for Pm4CL1 and PmSPS2

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Inserts

    Pm4CL1
    PmSPS2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGAP

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCoofy52

    Plasmid

    #122006

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-3xAvi

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWDY1211

    Plasmid

    #138591

    Purpose

    Expresses NatL2 in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. luxB:cp157Venus+luxA/pRSETB Duet

    Plasmid

    #197583

    Purpose

    BRET lux luciferase with cp157Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBcp157Venus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPAP004

    Plasmid

    #160911

    Purpose

    E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    lacZ selection cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC57-Curlcake3

    Plasmid

    #139342

    Purpose

    Expresses Curlcake3 (CC3) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake3 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC57-Curlcake1

    Plasmid

    #139340

    Purpose

    Expresses Curlcake1 (CC1) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake1 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC57-Curlcake2

    Plasmid

    #139341

    Purpose

    Expresses Curlcake2 (CC2) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake2 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC57-Curlcake4

    Plasmid

    #139343

    Purpose

    Expresses Curlcake4 (CC4) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake4 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. luxB:Venus+luxA/pRSETB Duet

    Plasmid

    #197582

    Purpose

    BRET lux luciferase with Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBVenus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBXNH3CA

    Plasmid

    #47069

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), AVI-tag, and…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSt-KO

    Plasmid

    #44566

    Purpose

    for generation of knockout of mycobacterial genes

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pIntegrase_9

    Plasmid

    #60580

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHA1

    Plasmid

    #85709

    Purpose

    This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

    Depositor

    Ulrich Koszinowski, Martin Messerle

    Article

    Adler et al J Virol. 2000 Aug;74(15):6964-74.

    Type

    Empty backbone

    Use

    Cre/Lox; Herpesvirus cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBadcrtEBI_13

    Plasmid

    #63811

    Purpose

    expresses crtEBI genes for neurosporene production

    Depositor

    Howard Salis

    Article

    Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

    Insert

    crtEBI operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    PBAD

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET30b-7Y-SrtA

    Plasmid

    #105601

    Purpose

    expresses 7Y sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7Y mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNH051

    Plasmid

    #42157

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    GFP-RT-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET21d-colEwt2/Imwt2

    Plasmid

    #107127

    Purpose

    plasmid for expression of WT colicin endonuclease and immunity pair 2

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEwt2/Imwt2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pBXCA3GH

    Plasmid

    #47071

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), Avi-tag, GFP…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET30b-7D-SrtA

    Plasmid

    #105600

    Purpose

    expresses 7D sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7D mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET29b-EPN01*-His

    Plasmid

    #84509

    Purpose

    E.coli version of EPN01*

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN01*-His_pET29b
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge