We narrowed to 12,555 results for: LIC

1. pDONR223_TCF7L2_WT

   Plasmid

   #81745

   Purpose

   Gateway Donor vector containing TCF7L2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_PRKCE_WT

   Plasmid

   #82227

   Purpose

   Gateway Donor vector containing PRKCE , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PRKCE (PRKCE Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_RBM11_WT

   Plasmid

   #81784

   Purpose

   Gateway Donor vector containing RBM11 ,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RBM11 (RBM11 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-3A2-mCherry

   Plasmid

   #73425

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3A2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3A2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Rabbit Cav 2.2 W391A pMT2

   Plasmid

   #58734

   Purpose

   expression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   Cav2.2 (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 (-) hYAP 1-2beta

   Plasmid

   #124144

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 (-) hYAP 1-1gamma

   Plasmid

   #124141

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV6-Entry IL36RN R102W

   Plasmid

   #58323

   Purpose

   entry vector for human IL36RN R102W mutant

   Depositor

   Francesca Capon

   Article

   Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

   Insert

   IL36RN (IL36RN Human)
   

   Use

   Entry vector

   Tags

   myc-DDK

   Expression

   Mutation

   R102W: c.304C>T, p.Arg102Trp​

   Promoter

   CMV

   Available since

   July 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1/NDUFS2 _34-78/myc-His

   Plasmid

   #127496

   Purpose

   Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 _34-78 (NDUFS2 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 _34-78

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_SLC2A3_p.F88S

    Plasmid

    #81473

    Purpose

    Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F88S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_SLC2A3_p.G109V

    Plasmid

    #81478

    Purpose

    Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G109V

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_SLC2A3_p.K49N

    Plasmid

    #81461

    Purpose

    Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    K49N

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_RGR_WT

    Plasmid

    #81845

    Purpose

    Gateway Donor vector containing RGR , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RGR (RGR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_RAB42_WT

    Plasmid

    #81796

    Purpose

    Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RAB42 (RAB42 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_hE2F7mg_2ntmut

    Plasmid

    #73074

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns, 2…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223_SGK1_p.A48G

    Plasmid

    #81430

    Purpose

    Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK1 (SGK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    A48G

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_SGK1_p.F27L

    Plasmid

    #81391

    Purpose

    Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK1 (SGK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F27L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_C13orf37_WT

    Plasmid

    #81872

    Purpose

    Gateway Donor vector containing MZT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    C13orf37 (MZT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S5G

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-5F5-mCherry

    Plasmid

    #73422

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 5F5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P5F5 (orthogonal T7-lac variant)

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti CGIP + M-AAT target

    Plasmid

    #86008

    Purpose

    lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    M-AAT target
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V30M

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-3_Flag-YTHDF2

    Plasmid

    #238975

    Purpose

    Expresses YTHDF2

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT

    Plasmid

    #218455

    Purpose

    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tag

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PSIN4-3_Flag-ATP5MG

    Plasmid

    #232951

    Purpose

    Expresses ATP5MG

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    ATP5MG (ATP5MG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH-3_Flag-ATP5MF

    Plasmid

    #232950

    Purpose

    Expresses ATP5MF

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    ATP5MF (ATP5MF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-3_Flag-ATP5PB

    Plasmid

    #232949

    Purpose

    Expresses ATP5PB

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    ATP5PB (ATP5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-Grik1-GFP

    Plasmid

    #233645

    Purpose

    Expresses GFP in OFF cone bipolar cells

    Depositor

    Connie Cepko

    Article

    Avilés et al J Gen Physiol. 2025 Mar 3;157(2):e202413

    Insert

    Grik1 promoter (Grik1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCR4_miniFXN_8

    Plasmid

    #234361

    Purpose

    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.

    Depositor

    Marek Napierala

    Article

    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

    Insert

    FXN (FXN Human)
    

    Use

    Cloning

    Tags

    Flag Tag

    Expression

    Mutation

    There is a partial deletion in the 5'UTR of …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid

    #229814

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid

    #229816

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid

    #229841

    Purpose

    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-H2B-mCerulean-2xStop-loxP
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Epac-S-H326

    Plasmid

    #228242

    Purpose

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Epac-S-H322

    Plasmid

    #228246

    Purpose

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Epac-S-H323

    Plasmid

    #228247

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Epac-S-H324

    Plasmid

    #228248

    Purpose

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Epac-S-H325

    Plasmid

    #228249

    Purpose

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Epac-S-H332

    Plasmid

    #228250

    Purpose

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187

    Insert

    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Human, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCA528 IST1 L328D, L355A (1-366)

    Plasmid

    #193040

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, L355A

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG CAPN7 V18D-OSF

    Plasmid

    #193042

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG CAPN7 L61D-OSF

    Plasmid

    #193043

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    L61D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAG CAPN7 F98D-OSF

    Plasmid

    #193044

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    F98D

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLVX-CAPN7-V18D-mCherry

    Plasmid

    #193046

    Purpose

    Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

    Promoter

    Tight TRE

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLVX-CAPN7-C290S-mCherry

    Plasmid

    #193047

    Purpose

    Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

    Promoter

    Tight TRE

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCA528 CAPN7 V18D,F98D (1-165)

    Plasmid

    #193034

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    V18D, F98D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCA528 IST1, residues 316-366, C-Cys

    Plasmid

    #191234

    Purpose

    Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 316-366, contains nonnative C-terminus f…

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCA528 CHMP4C 156-233

    Plasmid

    #108257

    Purpose

    Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C residues 156-233 (CHMP4C Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCA528 CHMP5 159-222

    Plasmid

    #108259

    Purpose

    Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP5 residues 148-219 (CHMP5 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge