12,323 results

1. U6b-T

   Plasmid

   #117210

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   u6b promoter-gRNA
   

   Use

   Synthetic Biology

   Expression

   Insect

   Promoter

   U6

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA_Level 2A

   Plasmid

   #102705

   Purpose

   Mobius Assembly Level 2 Acceptor Vector A

   Depositor

   Naomi Nakayama

   Article

   Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

   Insert

   J23106:B0034:sfGFP:rrnBT1-T7TE
   

   Use

   Synthetic Biology

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2903(XI-2 MarkerFree)

   Plasmid

   #73275

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-TL5

   Plasmid

   #72989

   Purpose

   RBS_M2

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   M2 RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pBP-TL11

   Plasmid

   #72995

   Purpose

   RBS_L4

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L4 RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAGM9121_pFMD

   Plasmid

   #160921

   Purpose

   Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   Formate dehydrogenase promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BbsI domesticated

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM9121_pCAT1

   Plasmid

   #160918

   Purpose

   Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   Catalase 1 promoter
   

   Use

   Synthetic Biology

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBK.014

   Plasmid

   #187215

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.R400

   Plasmid

   #138175

   Purpose

   Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbB8a-GFP

    Plasmid

    #35361

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSilencer2.1-U6-LRS1F50C

    Plasmid

    #190856

    Purpose

    For mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    LRS1F50C (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108849

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISP_IS

    Plasmid

    #120425

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB1_alpha2

    Plasmid

    #68225

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB1_omega1

    Plasmid

    #68234

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB1_omega2

    Plasmid

    #68235

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP43-2A(sfGFP)

    Plasmid

    #126987

    Purpose

    Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOG603

    Plasmid

    #105356

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor RPS5a (At3g11940; 1700bp)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL 233

    Plasmid

    #37754

    Depositor

    Han Lim

    Article

    Hussein et al Nucleic Acids Res. 2012 May 22.

    Inserts

    ptet_ompC_gfp
    pLac_st7 TetR_mCherry
    

    Use

    Synthetic Biology; E. coli

    Tags

    gfp and mCherry

    Promoter

    pLac and ptet

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only