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pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Use

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSuper-PACSIN1-shRNA

Plasmid

#72576

Purpose

Expresses shRNA against PACSIN1

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

PACSIN1 (Pacsin1 Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-MOR272-1

Plasmid

#22377

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR272-1 (Olfr167 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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mEos4b-Smoothened-N-18

Plasmid

#62780

Purpose

Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Smoothened

Use

Tags

mEos4b

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PB-TAC-MKOS

Plasmid

#80484

Purpose

piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.51.5

Plasmid

#169024

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.55.9

Plasmid

#169026

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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3392 pcDNA3 Bid p15

Plasmid

#8808

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p15 (Bid Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pISH-Olfr78

Plasmid

#105491

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr78 (Or51e2, MOR18-2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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TtGNN-TAF4-HFD

Plasmid

#106381

Purpose

dox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

GFP fused TAF4 HFD (827-909) (Taf4 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-Rheb-HVR HRas-IP

Plasmid

#13833

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb-HVR HRas (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminal of Rheb and hyper variable region (HVR…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
Anti-Brevican [N294A/10R]

Plasmid

#177528

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat). Derived from hybridoma N294A/10R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody (Bcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-NCKX4 [N414/25R]

Plasmid

#177549

Purpose

Mammalian Expression Plasmid of anti-NCKX4 (Human). Derived from hybridoma N414/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NCKX4 (Homo sapiens) recombinant mouse monoclonal antibody (SLC24A4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.2 K+ channel [N410/17R]

Plasmid

#177548

Purpose

Mammalian Expression Plasmid of anti-Kv3.2 K+ channel (Rat). Derived from hybridoma N410/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv3.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv2.1 K+ channel [L83/11R]

Plasmid

#177490

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma L83/11.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv1.2 K+ channel [L76/36R]

Plasmid

#177489

Purpose

Mammalian Expression Plasmid of anti-Kv1.2 K+ channel (Human). Derived from hybridoma L76/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv1.2 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNA2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv2.1 K+ channel [L61C/30R]

Plasmid

#177487

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma L61C/30.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18S-3HA-mULK2(K39T)

Plasmid

#22899

Depositor

Masaaki Muramatsu

Article

Yan et al Oncogene. 1999 Oct 21. 18(43):5850-9.

Insert

UNC-51-like kinase 2 (Ulk2 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only
pCI-MOR236-1

Plasmid

#22365

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR236-1 (Olfr1264 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
jk64

Plasmid

#87012

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nrx1B-4xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos3.2-Telethonin-N-18

Plasmid

#62771

Purpose

Localization: Scaffold, N1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580

Depositor

Michael Davidson

Insert

Telethonin

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

Plasmid

#105019

Purpose

Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps39 (Vps39 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-mIFITM3-deltaY20

Plasmid

#58459

Purpose

Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
XE245 Mouse Naked-2 CS2p+

Plasmid

#16727

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Mouse Naked-2 pCS2P+F (Nkd2 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

*

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCLXSN(GFP)-HA-Tbkbp1

Plasmid

#125177

Purpose

Retroviral expression of mouse Tbkbp1

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoD

Plasmid

#55700

Purpose

expression of mouse RhoD

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoD (Rhod Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pFIN-mIRBP156-CHER-RK-GFP-WPRE

Plasmid

#44593

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

IRBP156-CHER
RK-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human rhodopsin kinase (pos 3723-4019) and murine…

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-mTAZ-WW (86)

Plasmid

#40915

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ 14-3-3 binding site and WW domain (…

Promoter

Tac

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pUC19 PKC epsilon

Plasmid

#8442

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pRDAX-018 FLAG Venus210[1] IRES Venus210[2]

Plasmid

#130128

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-1003

Plasmid

#31712

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-367 (Mir367 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-CreERT2 sgRipk4 v3

Plasmid

#158048

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CBP-CH3-SP65

Plasmid

#21092

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Expression

Mutation

CH3 domain aa 1625-1877

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pGL5

Plasmid

#48745

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to +2064)

Use

Luciferase

Tags

Expression

Mutation

truncated promoter region (see pGL6)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pmiReport PU.1 3' UTR

Plasmid

#26530

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

PU.1 3' UTR (Spi1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5bHA

Plasmid

#18034

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5b (Wnt5b Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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