12,913 results

Showing: 6881 - 6920 of 12913 results

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SIN40C.SFFV.mArid3a.IRES.GFP

Plasmid

#169309

Purpose

Constitutive overexpression of murine Arid3a cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CBP-HAT-SP65

Plasmid

#21088

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

HAT domain aa 1172-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pPBpuro-mNG-eDHFR(69K6)-p85iSH2

Plasmid

#172103

Purpose

Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Clover-MyosinIIA-C-18

Plasmid

#56372

Purpose

Localization: Cytoskeleton, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MyosinIIA

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS/KS-mId4sp

Plasmid

#16046

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id4 (Id4 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 665-945

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
TrpV1 scFv [N221/17]

Plasmid

#190540

Purpose

Mammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-INPP4b [N171/17.4R]

Plasmid

#114551

Purpose

Mammalian Expression Plasmid of anti-INPP4b (Human). Derived from hybridoma N171/17.4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-INPP4b (Homo sapiens) recombinant mouse monoclonal antibody (INPP4B Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Sema3b(L)-AP-His

Plasmid

#72013

Purpose

Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 KSR1 S392A

Plasmid

#25976

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
binder/tag-WT-FAK-insertionA

Plasmid

#190439

Purpose

The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

FAK (Ptk2 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

SsrA inserted after aa 35

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-CrxshRNA

Plasmid

#73987

Purpose

Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Crx gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-GDNF

Plasmid

#119897

Purpose

Mouse GDNF In situ hybridization probe

Depositor

Wenqin Luo

Article

Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

Insert

glial cell line derived neurotrophic factor (Gdnf Mouse)

Use

In situ hybridization probe

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-MOR271-1

Plasmid

#22376

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR271-1 (Olfr168 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-AdenoX

Plasmid

#122123

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Use

Adenoviral

Tags

Omni

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
RCAS(A) mWnt1 (CT#165)

Plasmid

#14017

Depositor

Cliff Tabin

Insert

Wnt1 (Wnt1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Cntn4.2-Fc-His

Plasmid

#72068

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse ZP2 (JD#147)

Plasmid

#14645

Depositor

Jurrien Dean

Article

Liang et al Mol Cell Biol. 1990 Apr . 10(4):1507-15.

Insert

ZP2 (Zp2 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-dSa VP64 Neurog2

Plasmid

#99695

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)

Use

AAV and CRISPR

Tags

VP64

Mutation

dead Cas9

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-MOR256-17

Plasmid

#22369

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR256-17 (Olfr15 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-F-Gcn5-NLSm

Plasmid

#63708

Purpose

retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated K425, K427 and K438 to R (arginine)

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV_ACTB CRISPIE

Plasmid

#172853

Purpose

Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Inserts

Actb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRNATU6.1 HDAC3 shRNA2

Plasmid

#112271

Purpose

HDAC3 KD

Depositor

Simone Di Giovanni

Insert

shRNA against mouse HDAC3 (Hdac3 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
RRAS2

Plasmid

#55660

Purpose

expression of mouse RRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS2 (Rras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

Plasmid

#46581

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-10a Decoy (MIR10A, Mir10a Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway destination vector

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7-Casp2-shRNA

Plasmid

#157926

Purpose

Knockdown of caspase-2

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Casp2 shRNA (Casp2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Promoter

mouse U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

Plasmid

#177236

Purpose

Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgSnrk

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

Plasmid

#177234

Purpose

Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNuak1/sgNuak2

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

Plasmid

#177233

Purpose

Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgPrkaa1/sgPrkaa2

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd

Plasmid

#177230

Purpose

Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgLkb1_2nd

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

Plasmid

#177227

Purpose

Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgLkb1_1st

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-Trib1

Plasmid

#131156

Purpose

Mammalian expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Px458-Cas9n-Trp63-Exon4-2sgRNA

Plasmid

#88849

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63 (Trp63 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
mEmerald-TMEM216-N-18

Plasmid

#54280

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TMEM216 (Tmem216 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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