We narrowed to 17,013 results for: Por

1. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL2Rα-EGFP

   Plasmid

   #86055

   Purpose

   A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Interleukin-2 receptor subunit alpha (IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_LaG17-mCherry-HOTag3

   Plasmid

   #190024

   Purpose

   Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   LaG17 CluMPS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-FLEX-eNpHR-EYFP

   Plasmid

   #89876

   Purpose

   To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   eNpHR=EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL204

   Plasmid

   #22282

   Purpose

   Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. FL MALT1 WT in pET29b-Strep

   Plasmid

   #48968

   Purpose

   Expresses Full length wild type MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Tags

   His and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pQCXIP-SHMY-PAUF

   Plasmid

   #182645

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   Pancreatic adenocarcinoma upregulated factor (PAUF)
   

   Use

   Retroviral

   Tags

   His6-tag, N-terminal ER targeting sequence of hem…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Promoter

   U6

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pRR-ERalpha-5Z

    Plasmid

    #23061

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    ERalpha (ESR1 Human)
    

    Expression

    Yeast

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hSyn-DIO-GRAB_eCB2.0

    Plasmid

    #164606

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-NES-PguCas13b-msfGFP-NES-Flag

    Plasmid

    #165072

    Purpose

    overexpression of PguCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCerulean-VSVG

    Plasmid

    #11913

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Expression

    Mammalian

    Mutation

    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myc-βarrestin2-LOV-Turbo_pCDNA3

    Plasmid

    #199671

    Purpose

    expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Tags

    Myc and βarrestin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iRFP682-Smad2

    Plasmid

    #118943

    Purpose

    Mammalian expression of human Smad2 protein

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    iRFP682-Smad2 (SMAD2 Human)
    

    Tags

    iRFP682

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21 Catalytic Domain of MALT1

    Plasmid

    #48970

    Purpose

    Expresses catalytic domain of MALT1 in e.coli AA 329-566

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 (MALT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains AA 329-566

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLHCX-FLAG-mPKM1

    Plasmid

    #44240

    Depositor

    Matthew Vander Heiden, Lewis Cantley

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M1 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGEXtev-HMG54kd

    Plasmid

    #26030

    Depositor

    Johann Deisenhofer

    Article

    Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

    Insert

    3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Catalytic domain (amino acids 426-488) Methion…

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.MARK2-Halo

    Plasmid

    #224414

    Purpose

    Mammalian expression of MARK2

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    MARK2 (MARK2 Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    Chicken Beta actin

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-GfaABC1D-GRAB_Ado1.0med

    Plasmid

    #153289

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SLC39A14-GFP

    Plasmid

    #104380

    Purpose

    Expresses SLC39A14/ZIP14 wild-type in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRE-UBC-HLA-G SCT-T2A-GFP

    Plasmid

    #205462

    Purpose

    lentiviral plasmid for expression of HLA-G SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-G SCT (HLA-G Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid

    #205540

    Purpose

    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TRE3GS

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLIK 3xFLAG-CSE1L zeo

    Plasmid

    #192320

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a SUMO Nup53xl 1-320

    Plasmid

    #85998

    Purpose

    bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG/hArf4(WT)-mCherry

    Plasmid

    #79406

    Purpose

    Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

39. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Expression

    Yeast

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge