We narrowed to 11,805 results for: NSI;

Showing: 7001 - 7040 of 11805 results

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pAAV-CamKII-ArchT-GFP

Plasmid

#37807

Purpose

Viral expression of ArchT-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-T1977R

Plasmid

#69011

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-T1977R (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Threonine 1977 to Arginine

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2 sgBLM10

Plasmid

#127643

Purpose

Knock-out of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

Plasmid

#101300

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-509, 670-698, and 699-906 …

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSW505 - pOp4:3xGFP-NLS

Plasmid

#116005

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR

Tags

linker-NLS

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Expression

Bacterial

Promoter

J23108 and PpsdA110

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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MEIS1A-MIY

Plasmid

#21013

Depositor

Corey Largman

Article

Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

Insert

MEIS1A (Meis1 Mouse)

Use

Retroviral

Tags

IRES and YFP

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
NSK026 CMV-TO-p53R248W (FLP-IN)

Plasmid

#191168

Purpose

Expression of human p53 mutant R248W in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

p53(R248W) (TP53 Human)

Expression

Mammalian

Mutation

R248W

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV neo WT Akt1

Plasmid

#14549

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_HCLS1-1/1

Plasmid

#91437

Purpose

Protein expression and purification of human SH3 domain construct HCLS1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

HCLS1-1/1 (HCLS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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His-SUMO- flag-hDDI2-D252N_pET11a

Plasmid

#169123

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-KD

Plasmid

#140297

Purpose

Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSW648 - pREV>>

Plasmid

#115996

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-mAvicFP1

Plasmid

#129511

Purpose

Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

H2B-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 fused to the C terminus of human histone…

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-mAvicFP1

Plasmid

#129509

Purpose

Expresses mAvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

mAvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

Plasmid

#61841

Purpose

mammalian expression of cyclin B1 non-degradable L45A mutant

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

cyclin B1 (CCNB1 Human)

Tags

2xSTREP and 3xFLAG

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-B2AR-APEX

Plasmid

#96848

Purpose

For expression of B2AR-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

B2AR-APEX (ADRB2 Human)

Expression

Mammalian

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR3c-R248C

Plasmid

#45712

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R248C

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only
EGFP-Orf9b L52D

Plasmid

#165129

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf9b L52D

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn1_AchLightR

Plasmid

#187463

Purpose

Expresses red acetylcholine indicator AchLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightR

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100856

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_COX41

Plasmid

#111664

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

COX41 (COX4I1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV neo KD Akt1

Plasmid

#14550

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt1 KD (Akt1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Kinase Dead Mutant: K179M

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-C1-FKBP-CYB5tail

Plasmid

#202686

Purpose

rapamycin-induced recruitment of proteins to ER

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_HXK1

Plasmid

#111668

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

HXK1 (HK1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_CSK-1/1

Plasmid

#91542

Purpose

Protein expression and purification of human SH3 domain construct CSK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CSK-1/1 (CSK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBp-FGFR3c-S249C

Plasmid

#45713

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S249C

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPc

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 7001 - 7040 of 11805 results

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We narrowed to 11,805 results for: NSI;