159,461 results

1. Tg(mpeg1:LifeAct-mRuby)

   Plasmid

   #166977

   Purpose

   labels F-actin in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

   Inserts

   mpeg1 (mpeg1.1 Zebrafish)
   LifeAct (actb1 Frog)
   

   Use

   Tags

   Lifeact-mRuby and mRuby

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-hSyn-RiboL1-jGCaMP8s

   Plasmid

   #169247

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW57.1-4EBP1_4xAla

   Plasmid

   #38240

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   T37A, T46A, S65A, T70A (dominant negative)

   Promoter

   Tet ON

   Available since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 GFP LIC cloning vector (6D)

   Plasmid

   #30127

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 1227 pcDNA3 Myr HA Akt2

   Plasmid

   #9016

   Depositor

   William Sellers

   Insert

   Akt2 (AKT2 Human)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

   Plasmid

   #154850

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LZ10 PBREBAC-H2BHalo

   Plasmid

   #91564

   Purpose

   piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

   Depositor

   James Zhe Liu

   Article

   Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

   Insert

   histone H2B-HaloTag
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCardinal-pBAD

   Plasmid

   #54800

   Purpose

   Localization: Protein Expression Vector , Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   6xHis and mCardinal

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJR98

    Plasmid

    #187239

    Purpose

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPSU250

    Plasmid

    #173837

    Purpose

    Expresses 250 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV-RFP

    Plasmid

    #26001

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-RFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge