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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-c-Myc-IP

Plasmid

#15921

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Use

Tags

MBP-6xHis-NLS

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTgenomic

Plasmid

#1778

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

Plasmid

#51084

Purpose

Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV Retrograde and AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Mutation

Promoter

human Synapsin1

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pEB-Tg

Plasmid

#28220

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

SV40 large T

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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GolgiCFP

Plasmid

#14873

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

CFP

Use

Tags

eNOS(1-35)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP36

Plasmid

#22579

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP36 (USP36 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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P210 pcDNA3

Plasmid

#27481

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (ABL1, BCR Human)

Use

Tags

Expression

Mammalian

Mutation

Contains the complete bcr/abl fusion

Promoter

CMV

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001 . 1():4.

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ER beta

Plasmid

#28237

Depositor

Michael Mancini

Insert

ER beta (ESR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
Reduced Expression GFP beta actin

Plasmid

#31502

Depositor

Tim Mitchison, Rick Horwitz

Article

Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

Insert

beta actin (ACTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

91-544 of enhancer region of CMV promoter deleted

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCherry-mNrp1

Plasmid

#21934

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Tags

Cherry and His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
pH3U3-mcs

Plasmid

#12609

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Multiple cloning site in His3 Ura3 reporter plasmid

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pHCMV-RabiesG

Plasmid

#15785

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA8

Plasmid

#19487

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA8 (HSPA8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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Cherry-LacRep

Plasmid

#18985

Depositor

Mirek Dundr

Article

Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

Insert

LacI

Use

Tags

AviTag, SV40 NLS, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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EF.hHES1.Ubc.GFP

Plasmid

#17624

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCFJ356 - MCS(cxTi10816, IV)

Plasmid

#34871

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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