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Showing: 5281 - 5310 of 28391 results

  1. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  2. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid
    #124399
    Purpose
    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Shaun Cowley
    Article
    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.
    Insert
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/…
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124399-plasmid-map-sequence-id-245260

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  7. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  9. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid
    #176149
    Purpose
    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    X-ray repair cross complementing 1 (XRCC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…
    Promoter
    CMV
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176149-plasmid-map-sequence-id-353190

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  11. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    174554-plasmid-map-sequence-id-354966

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  14. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

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  19. Tom20-mChF-AIP(TE)

    Plasmid
    #61522
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TE mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pMSCVpuro-F-Gcn5-NLSm

    Plasmid
    #63708
    Purpose
    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
    Depositor
    Kai Ge
    Article
    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:
    Insert
    Gcn5 (Kat2a Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    mutated K425, K427 and K438 to R (arginine)
    Promoter
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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Showing: 5281 - 5310 of 28391 results