We narrowed to 18,334 results for: met
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Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPK1169: CAG-DmrC-NLS-3xFLAG-P65
Plasmid#104564PurposeMammalian expression vector for DmrC fused to p65 activation domainDepositorInsertDmrC-p65
UseTags3x FLAG and NLSExpressionMammalianMutationPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Clover-pBAD
Plasmid#54575PurposeLocalization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.DepositorTypeEmpty backboneUseTags6xHis and CloverExpressionBacterialMutationPromoterAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Str-KDEL_ST-SBP-tagBFP
Plasmid#65266Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-PCAF-C2
Plasmid#134572PurposeExpresses YFP-PCAF fusion proteinDepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-Puro
Plasmid#84540PurposeCRISPR - FLIP targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-FlpE-Puro
Plasmid#84541PurposeCRISPR - FLIP-FlpE targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP-FlpE - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE
Plasmid#136916PurposeExpresses ChR2(H134R)-EYFP in Cre-negative cellsDepositorInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationH134R in ChR2PromoterHuman Synapsin1Available SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only