We narrowed to 78,797 results for: TRI

1. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA1-Bystin

   Plasmid

   #11869

   Depositor

   Michiko Fukuda

   Article

   Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

   Insert

   Bystin (BYSL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pEN_TT JunD-HA

   Plasmid

   #58498

   Purpose

   Gateway entry vector for an inducible HA-tagged mouse JunD

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JunD (Jund Mouse)
   

   Use

   Entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMXs-hs-3xHA-deltaRING-LIN-41

   Plasmid

   #52718

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 12-91

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. FH1-PNGT#1-mCherry

   Plasmid

   #164098

   Purpose

   Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   PNGT#1-mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1

   Plasmid

   #45822

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   AS9(Mito MTS)-Delta N67-mMCL1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ATP Synthase subunit 9 is used as a Mito Targetin…

   Promoter

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

   Plasmid

   #129531

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXB7 (human) pCMV

   Plasmid

   #8539

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB7 (HOXB7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-C3-PLK4-S305E-3xFLAG

    Plasmid

    #69840

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S305E

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid

    #69841

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S285A/T289A

    Promoter

    CMV

    Available since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS3-MT-BX/ARF1-64

    Plasmid

    #78762

    Purpose

    To overexpress ARF1-64 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    1-64

    Promoter

    CMV IE94

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCS3-MT-BX/ARF65-132

    Plasmid

    #78763

    Purpose

    To overexpress ARF65-132 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    65-132

    Promoter

    CMV IE94

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-SwitchON-sgCh2-2

    Plasmid

    #199637

    Purpose

    Tamoxifen-inducible expression of sgRNA control targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRESneo-MPG(N169D)

    Plasmid

    #23259

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan 12. ():.

    Insert

    N-methylpurine DNA glycosylase (N169D) (MPG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N169D

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Use

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS2044

    Plasmid

    #79652

    Purpose

    ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple255-EmGFP WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PAX6 HS234Z Enhancer

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80056

    Purpose

    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    flk1

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HH06-LP Clone 6 heavy chain

    Plasmid

    #192176

    Purpose

    Clone 6 heavy chain (HH06)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HH02-LP Clone 2 heavy chain

    Plasmid

    #192175

    Purpose

    Clone 2 heavy chain (HH02)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HK02-LP Clone 2 light chain

    Plasmid

    #192179

    Purpose

    Clone 2 light chain (HK02)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 light chain (HK02)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HOXB4 (human) FLAG MSCV

    Plasmid

    #8528

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Missing N-terminal 14 amino acids; improved Ribos…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-hGRM1-RFP

    Plasmid

    #22920

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-mA93-RFP

    Plasmid

    #22918

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-6P-1-INTS3-N

    Plasmid

    #128416

    Purpose

    Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal region (1–513 aa)

    Promoter

    tac promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jean-Loup Faulon, Jerome Bonnet

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only