We narrowed to 5,897 results for: pcDNA 3.1

1. alpha catenin conformation sensor

   Plasmid

   #71709

   Purpose

   This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin FRET Sensor
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-MED18

   Plasmid

   #15419

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED18 (MED18 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. ER-mGreenLantern

   Plasmid

   #164463

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. F-MED26

   Plasmid

   #15367

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED26 (MED26 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. LUC01 (L/Sec/P)

   Plasmid

   #112144

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U)

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSI-Target-AID-NG

   Plasmid

   #119861

   Purpose

   Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LUC05 (L/Cys/P)

   Plasmid

   #112146

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-MED7

   Plasmid

   #15416

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED7 (MED7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pAAV_OHu19986C_myc tag

   Plasmid

   #183174

   Purpose

   used to make AAV vectors for hsTRPA1 tagged with myc

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Insert

   hsTRPA1 (TRPA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Arrb1-RFP

    Plasmid

    #35401

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. tau exon 9–12 splicing minigene

    Plasmid

    #121165

    Purpose

    Generates circular RNAs from a Tau minigene

    Depositor

    Stefan Stamm

    Article

    Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

    Insert

    Tau exons 9–12 (MAPT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mRFP-human APPL1

    Plasmid

    #22202

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCD4-TagRFP-T (RHPMP151)

    Plasmid

    #119238

    Purpose

    Expresses CD4 fused to TagRFP-T

    Depositor

    Ricardo Henriques

    Article

    Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    tagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Mito-mTurq2-Apollo-NADP+

    Plasmid

    #193303

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2 and mitochondrial targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-hIes2

    Plasmid

    #15355

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    PAP-1 binding protein (INO80B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

19. P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

    Plasmid

    #202760

    Purpose

    Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    huAsCpf1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cyto-mTurq2-Apollo-NADP+

    Plasmid

    #193301

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SpyTag-RBD

    Plasmid

    #159999

    Purpose

    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Rlucrarr3-Flash6

    Plasmid

    #74134

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

25. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Opto-GPR55

    Plasmid

    #106026

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR55 (GPR55 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PH Akt-Venus

    Plasmid

    #85223

    Purpose

    Venus tagged PH domain of Akt for use as a PIP3 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    PH AKT(1-148) (AKT1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HiBiT-FZD5

    Plasmid

    #195843

    Purpose

    Expresses human FZD5 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD5 (FZD5 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. VN-Sph1

    Plasmid

    #89755

    Purpose

    expresses human Synphilin fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

    Insert

    SNCAIP (SNCAIP Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Rlucrarr3-Flash1

    Plasmid

    #74129

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only