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Showing: 681 - 720 of 847 results

  2. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

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  3. pCIFR-5

    Plasmid
    #233294
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    p14G-BCD2
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233294-plasmid-map-sequence-id-468478

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  4. pCIFR-3

    Plasmid
    #233295
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    p14G
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233295-plasmid-map-sequence-id-468475

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  5. pCIFR-6

    Plasmid
    #227665
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    p14G-BCD2
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227665-plasmid-map-sequence-id-458898

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  6. pCIFR-4

    Plasmid
    #227664
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    p14G-BCD2
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227664-plasmid-map-sequence-id-458897

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  7. pCIFR-2

    Plasmid
    #227663
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    p14G-BCD2
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227663-plasmid-map-sequence-id-458896

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  19. pME_G_0_3a_001_Placeholder

    Plasmid
    #235853
    Purpose
    Encodes a sfGFP cassette in the 3a position
    Depositor
    Tobias Erb
    Insert
    3a placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235853-plasmid-map-sequence-id-471454

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  22. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102882-plasmid-map-sequence-id-199289

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  25. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Expression
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  27. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  28. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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  29. pJ02B2Gm:Rm_AF

    Plasmid
    #66061
    Purpose
    MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    FACS Controls
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66061-plasmid-map-sequence-id-257697

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  30. pJ02B2Rm:Gm_AF

    Plasmid
    #66062
    Purpose
    MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    FACS Controls
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66062-plasmid-map-sequence-id-257698

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  33. pTEI128

    Plasmid
    #126209
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary, module 1
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126209-plasmid-map-sequence-id-246907

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  34. pTEI129

    Plasmid
    #126210
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary, module 1
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126210-plasmid-map-sequence-id-246910

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  36. pTEI177

    Plasmid
    #126077
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP1-10
    Use
    Binary, module 1
    Tags
    linker (GTGGGGS)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126077-plasmid-map-sequence-id-246860

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  37. pRUSH

    Plasmid
    #54479
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pOCC93

    Plasmid
    #118911
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118911-plasmid-map-sequence-id-232347

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  40. pOCC92

    Plasmid
    #118910
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    118910-plasmid-map-sequence-id-234552

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Showing: 681 - 720 of 847 results