We narrowed to 1,203 results for: Lipi

1. siRNA-resistant ABHD16A-S355A-mNG

   Plasmid

   #195167

   Purpose

   mammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. siRNA-resistant ABHD16A-H189AD194A-Halo

   Plasmid

   #195168

   Purpose

   mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   Halo tag

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. siRNA-resistant ABHD16A-S355A-Halo

   Plasmid

   #195169

   Purpose

   mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   Halo tag

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGS_591

   Plasmid

   #160653

   Purpose

   Expresses OLE1 under the control of the yeast Sup35 promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   YGL055W (OLE1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Sup35 promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mKate2-DrCavin1b in pT3TS-Dest

   Plasmid

   #194295

   Purpose

   In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   cavin1b (cavin1b Zebrafish)
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ABHD2-3'UTR-WT

   Plasmid

   #190094

   Purpose

   examine the activity of ABHD2 3'UTR

   Depositor

   Bowen Liu

   Article

   Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

   Insert

   ABHD2 3' UTR Wild Type (ABHD2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NmMetQ C20A

   Plasmid

   #172112

   Purpose

   Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

   Depositor

   Douglas Rees

   Article

   Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

   Insert

   Methionine Binding Protein
   

   Use

   Tags

   10x Histidine Tag and N.meningitidis signal seque…

   Expression

   Bacterial

   Mutation

   Changed Proline 22 to Alanine on MetQ and Cystein…

   Promoter

   T7 Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQlinkG2-PTPIP51(236-470)

   Plasmid

   #170534

   Purpose

   Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQlinkH-PTPIP51(236-470)

   Plasmid

   #170530

   Purpose

   Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCu-His6-Atg13[571–700]-Ss(424)

    Plasmid

    #166852

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-N1 Dynamin1 R290A D291A T292A

    Plasmid

    #112103

    Purpose

    Mammalian expression of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R290A D291A T292A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1 Dynamin1 T292A L293A P294A

    Plasmid

    #112104

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T292A L293A P294A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N1 Dynamin1 L330R Q334R L702R

    Plasmid

    #112105

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L330R Q334R L702R

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-N1 Dynamin1 D406A M407A

    Plasmid

    #112107

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406A M407A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-N1 Dynamin1 D406A M407A F409A

    Plasmid

    #112108

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406A M407A F409A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1 Dynamin1 D406R M407R

    Plasmid

    #112109

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406R M407R

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N1 Dynamin1 D406R M407R F409A

    Plasmid

    #112110

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406R M407R F409A

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1 Dynamin1 D406R M407R T488W

    Plasmid

    #112111

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406R M407R T488W

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCD1E

    Plasmid

    #102956

    Purpose

    gene cloning of human CD1E

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

    Insert

    CD1E cDNA (CD1E Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

    Plasmid

    #65846

    Purpose

    Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

    Plasmid

    #65847

    Purpose

    Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKeima-LiveDrop

    Plasmid

    #199695

    Purpose

    lipophagy reporter system based on the Keima-fluorophore

    Depositor

    Tobias Walther

    Article

    Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

    Insert

    GPAT4 (GPAT4 Human)
    

    Use

    Tags

    Keima

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA6-N-Myc-SCAP

    Plasmid

    #134285

    Purpose

    Expresses Myc-tagged SCAP in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SCAP (Scap, Scap Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHCD1C

    Plasmid

    #102954

    Purpose

    gene cloning of human CD1C

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

    Insert

    CD1C cDNA (CD1C Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA6-N-Flag-SREBP2

    Plasmid

    #134284

    Purpose

    Expresses Flag-tagged SREBP2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-GRAM1b

    Plasmid

    #211494

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

    Plasmid

    #116876

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJM1-PM-RA-BlastR

    Plasmid

    #211708

    Purpose

    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    PM-targetted RA (Lck Synthetic)
    

    Use

    Lentiviral

    Tags

    RA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid

    #62373

    Purpose

    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    

    Use

    Tags

    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

    Expression

    Bacterial

    Mutation

    Y67S amino acid substitution in (-30)GFP renders …

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMRXIP-GFP-hATG2A

    Plasmid

    #114462

    Purpose

    Expresses human ATG2A with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

    Insert

    human ATG2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P656R (please see depositors comment below)

    Promoter

    CMV

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PMP34-mApple

    Plasmid

    #214417

    Purpose

    Peroxisome localization of PMP34 fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQCXIP mScarlet-MOSPD2 WT

    Plasmid

    #186472

    Purpose

    Expression of human MOSPD2 fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-2xPH-TAPP1 R211L mutant

    Plasmid

    #161991

    Purpose

    Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R211L

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMDC43-FIT2

    Plasmid

    #96993

    Purpose

    Express GFP-tagged mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    E2D (please see depositor comments below)

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCLHCX-mPM20D1-flag

    Plasmid

    #84566

    Purpose

    mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

    Depositor

    Bruce Spiegelman

    Article

    Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

    Insert

    peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    Retroviral

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

    Plasmid

    #169079

    Purpose

    Human ATG3

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6X Histidine Tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-Sec22b-shR

    Plasmid

    #208358

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations introduced to render shRNA …

    Promoter

    CMV

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Mouse Alpha2 delta-2 HA pMT2

    Plasmid

    #58731

    Purpose

    expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pX330-MGAT1-KO

    Plasmid

    #80009

    Purpose

    gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    MGAT1 (MGAT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Cav3P104L-YFP

    Plasmid

    #68404

    Purpose

    Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells

    Depositor

    Rob Parton

    Article

    Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.

    Insert

    Cav3 (Cav3 Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Proline at position 104 changed to a Leucine

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

48. M3-PSCFP2

    Plasmid

    #114185

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only