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We narrowed to 1,695 results for: SUA

Showing: 851 - 900 of 1695 results

  1. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

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  4. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84635-plasmid-map-sequence-id-176207

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  16. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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  18. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  19. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  20. ROZA-XL YF

    Plasmid
    #64195
    Purpose
    Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence
    Depositor
    Annemarie Lellouch
    Article
    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.
    Insert
    ROZA-XL YF
    Use
    Tags
    Expression
    Mammalian
    Mutation
    ROZA-XL tyrosine phosphorylation site mutated to …
    Promoter
    CMV
    Available Since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pcDNA3-mCherry-LKB1(S431A)

    Plasmid
    #84641
    Purpose
    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    mCherry-LBK1(S431A) (STK11 Synthetic, Human)
    Use
    Tags
    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Mutation
    PKA phosphorylation site Serine 428 mutated to Al…
    Promoter
    CMV
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    84641-plasmid-map-sequence-id-176205

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  23. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  24. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  25. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  42. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available Since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    1817-plasmid-map-sequence-id-350820

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  43. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid
    #216124
    Purpose
    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216124-plasmid-map-sequence-id-428729

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  50. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid
    #98929
    Purpose
    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98929-plasmid-map-sequence-id-190089

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Showing: 851 - 900 of 1695 results