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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
aav-tnt-actn2-gfp

Plasmid

#165034

Purpose

AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

ACTN2 (Actn2 Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

Plasmid

#89570

Purpose

Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

Depositor

McLean Bolton

Article

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Inserts

Channelrhodopsin
Histone 2B

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His-SUMO1

Plasmid

#159323

Purpose

Mammalian cell expression of mouse His-SUMO1

Depositor

Jianjun Zhao

Insert

His-SUMO1 (Sumo1 Mouse)

Expression

Mammalian

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

Plasmid

#182896

Purpose

Mammalian expression of TH mouse mAb HC gene

Depositor

Heng Zhu

Insert

anti-TH (human) heavy chain (TH Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

No

Promoter

CMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kir6.1 K+ channel [N366/60R]

Plasmid

#188206

Purpose

Mammalian Expression Plasmid of anti-Kir6.1 K+ channel (Rat). Derived from hybridoma N366/60

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir6.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnj8 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Foxo1

Plasmid

#118588

Purpose

Expresses Foxo1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Foxo1 (Foxo1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcdna3mciap1

Plasmid

#11474

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1 (Birc2 Mouse)

Expression

Mammalian

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-133a-1

Plasmid

#26326

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-133a-1 (Mir133a-1 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2-sgMaob-HepmCherry

Plasmid

#192829

Purpose

Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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p350_LTJ_180bpCD90.1Template

Plasmid

#104988

Purpose

180bp HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

180bp genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Hb9

Plasmid

#32930

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Hb9 (Mnx1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pKAM-E-Flag-GFP

Plasmid

#101866

Purpose

Lentiviral Evi-1-Flag with GFP marker

Depositor

Archibald Perkins

Insert

Evi-1 (Mecom Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 HA pMT2

Plasmid

#58731

Purpose

expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

Depositor

Annette Dolphin

Article

Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim5UTR-renilla

Plasmid

#38237

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim 5'UTR / Renilla (Vim Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Vim 5' UTR fused to renilla ORF

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-talin1 rod

Plasmid

#32855

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin 1 Rod aa 434-2541 (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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