164,722 results

1. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #125692

   Purpose

   Global labeling of inhibitory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PIEZO1-HaloTag

   Plasmid

   #207834

   Purpose

   PIEZO1 fusion for biochemistry and live imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   PIEZO1 (PIEZO1 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVS1

   Plasmid

   #33143

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Insert

   LacO (256 copies)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pGreenFire1_E2F1RE

   Plasmid

   #112248

   Purpose

   expression of GFP and Luciferase under the control of E2F1 responsive element

   Depositor

   Simone Di Giovanni

   Insert

   E2F1RE (responsive element)
   

   Use

   Lentiviral

   Tags

   copGFP and luciferase

   Expression

   Mutation

   Promoter

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIB-FLAG(N)-RPB1

   Plasmid

   #231722

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Fis1

   Plasmid

   #182580

   Purpose

   Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Fis1
   

   Use

   Tags

   Fis1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 8xNFAT-ZsG-hCD4

   Plasmid

   #162746

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA-MS2x2-TagRFP657

   Plasmid

   #184441

   Purpose

   sgRNA with MS2 aptamer, TagRFP657 as selection marker

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   sgRNA-MS2x2, TagRFP657
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-CreERT2

   Plasmid

   #139547

   Purpose

   CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   CreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK462 (Hygro-P2A-mAB)

    Plasmid

    #214388

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProEx-HTa-SOD1 WT

    Plasmid

    #191849

    Purpose

    express wild-type SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-FOXC1

    Plasmid

    #153114

    Purpose

    Expression of Flag tagged human FOXC1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXC1 (FOXC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn1-FLEX-CybSEP2-WPRE

    Plasmid

    #207661

    Purpose

    Monitoring neuropeptide release

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Cytochrome b-561 (Cyb561 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted 2 X SE…

    Promoter

    hSynapsin

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN239_CRISPRcharm_Kv1

    Plasmid

    #220838

    Purpose

    CRISPRcharm Kv1 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv1
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid

    #186052

    Purpose

    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV8

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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