We narrowed to 167,150 results for: Gene

Showing: 9101 - 9150 of 167150 results

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pEn-C1.1

Plasmid

#61479

Purpose

Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

Depositor

Holger Puchta

Article

Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-14-3-3 Beta

Plasmid

#19957

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

14-3-3 Protein Beta (YWHAB Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pSG424 Smad2 (Gal4-Smad2)

Plasmid

#14932

Depositor

Joan Massague

Article

Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

Insert

Smad2 (SMAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
p119 (FUGW-UbCp-rtTA3)

Plasmid

#105171

Purpose

ubiquitin C promoter drives rtTA3 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

UbCp-rtTA3

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
KX002: pMVP/PB-DEST

Plasmid

#121869

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHES941

Plasmid

#87942

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GAL4 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Synthetic, Human)

Expression

Yeast

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-DTA-DEST-R3R4(R)

Plasmid

#141011

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

DTA

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEE531

Plasmid

#149290

Purpose

T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPH1

Plasmid

#177662

Purpose

Recombinant protein synthesis of human HSPH1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPH1 (HSPH1 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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PROM5_35Sx2

Plasmid

#136112

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35Sx2 (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PROM5_35S

Plasmid

#136111

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMXs-PT2A-GFP-iRFP670-Tdtomato

Plasmid

#111813

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
iRFP670
Tdtomato

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd7-1D4

Plasmid

#42269

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 7 (Fzd7 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
PFKL_pCSdest

Plasmid

#53901

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PFKL (PFKL Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-Flag-YAP-S94/F95A

Plasmid

#33057

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S94A, F95A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mOtop3_pcDNA3

Plasmid

#114679

Purpose

This construct was used to express mouse Otop3 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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MRAP_pCSdest

Plasmid

#53833

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MRAP (MRAP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-v4.1

Plasmid

#204636

Purpose

Expression of AsCas12f v4.1 in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f-v4.1

Tags

NLS

Expression

Mammalian

Mutation

D169K, N199K, N328G, D364R

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMN-018

Plasmid

#195422

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pG3B-U6SC

Plasmid

#134753

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-35SC

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCVhyg-F-Axin1

Plasmid

#24928

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Axin1 (AXIN1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2010

Availability

Academic Institutions and Nonprofits only
pTK_BsmBI_LacZ_Citrine_Nanotag_4

Plasmid

#130514

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pANTO5

Plasmid

#131106

Purpose

To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

tetR gene by pFRA61 (Boldrin et al 2010)

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pCGN-ELK-1 Ets mut

Plasmid

#27157

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R62E/R65E/Y66D

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTK_BsmBI_LacZ_Cherry_Nanotag_105

Plasmid

#130546

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pG3B-U3Ub

Plasmid

#134750

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SLC5A3_pCSdest

Plasmid

#53836

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SLC5A3 (SLC5A3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pDONR221-kcnk3a

Plasmid

#208985

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3a (kcnk3a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KSTbx2.8.2

Plasmid

#42626

Depositor

Virginia Papaioannou

Article

Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

Insert

Tbx2 (Tbx2 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH4

Plasmid

#52529

Purpose

serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

2x FLAG-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-nDsRedFL-miR124

Plasmid

#177806

Purpose

The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-GFP-Bub1-S492Y

Plasmid

#16625

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 S492Y (BUB1 Human)

Expression

Mammalian

Mutation

S492Y

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMLS791

Plasmid

#188889

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ-440

Plasmid

#195464

Purpose

Lentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: KRAB-ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - KRAB - ZF3 - ERT2 - SMASh

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 167,150 results for: Gene