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We narrowed to 13,043 results for: SHI

Showing: 9101 - 9150 of 13043 results

  1. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

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  3. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

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  21. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169743
    Purpose
    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    mScarlet-I
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169743-plasmid-map-sequence-id-334355

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  30. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid
    #169734
    Purpose
    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169734-plasmid-map-sequence-id-334141

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  31. PB-TAC-OKMS-KRAB-dCas9

    Plasmid
    #120356
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120356-plasmid-map-sequence-id-236039

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  32. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

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  33. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159852
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159852-plasmid-map-sequence-id-326620

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  34. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid
    #159838
    Purpose
    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159838-plasmid-map-sequence-id-317992

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  35. pCIS-rPALcc

    Plasmid
    #145804
    Purpose
    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
    Depositor
    Betty Eipper, Richard Mains
    Article
    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed serine 767 to alanine to prevent glycosyl…
    Promoter
    CMV
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145804-plasmid-map-sequence-id-299336

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  36. iSKetSnFR2

    Plasmid
    #140467
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR2
    Use
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140467-plasmid-map-sequence-id-296110

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  37. iSKetSnFR1

    Plasmid
    #140466
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR1
    Use
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140466-plasmid-map-sequence-id-296106

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  38. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Ruma Banerjee
    Article
    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d
    Inserts
    mutA
    mutB
    Use
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    138296-plasmid-map-sequence-id-292603

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  43. pCEP4-HA-Flag-natT1R2 d835

    Plasmid
    #113959
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-834
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113959-plasmid-map-sequence-id-215288

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Showing: 9101 - 9150 of 13043 results