We narrowed to 88,655 results for: Mal;

1. pEGFP-2xFYVE

   Plasmid

   #140047

   Purpose

   Expresses GFP-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available Since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-Ubiquitin-K33

   Plasmid

   #17607

   Purpose

   Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K33 only, other lysines mutated to arginines; P19…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV_EF1a_CDK4-P2A-Hygro_Barcode

   Plasmid

   #170223

   Purpose

   Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-hGal3

   Plasmid

   #73080

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin-3 (LGALS3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_NRL_P2A_Hygro_Barcode

   Plasmid

   #120469

   Purpose

   Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NRL (NRL Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fuw-dCas9-Dnmt3a-P2A-tagBFP

    Plasmid

    #84569

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUGW-RUSH-LAMP1-V5-APEX2

    Plasmid

    #228524

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PD-1-miSFIT-Ce67

    Plasmid

    #124680

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS2 (hANGPTL4-V5)

    Plasmid

    #102446

    Purpose

    Expresses V5-tagged human ANGPTL4 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    ANGPTL4 (ANGPTL4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. peGFP C2-IST1

    Plasmid

    #218582

    Purpose

    Expresses GFP-tagged human IST1 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    IST1 (IST1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and Ef1A

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mLama1 AAV sgRNA 1, 2, 5

    Plasmid

    #135339

    Purpose

    Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    Lama1 VP64-dCas9 sgRNA Guides
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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