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We narrowed to 4,886 results for: phy

Showing: 3641 - 3660 of 4886 results

  2. pIYN3

    Plasmid
    #197099
    Purpose
    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.
    Depositor
    Kensaku Sakamoto
    Article
    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;
    Insert
    TyrRS variant, Nap3 tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Ala70, Thr158, Ser159, Arg286 (TyrRS)
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    197099-plasmid-map-sequence-id-387837

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  4. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139086-plasmid-map-sequence-id-273882

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  7. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174643
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(nano)-GFP
    Expression
    Mammalian
    Mutation
    EGFP: Met1Del; ppKin14VIb(aa861-1321)
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174643-plasmid-map-sequence-id-346994

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  9. pSW646 - pML1>>

    Plasmid
    #115995
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115995-plasmid-map-sequence-id-227319

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  10. pSW648 - pREV>>

    Plasmid
    #115996
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115996-plasmid-map-sequence-id-242815

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  11. pSW505 - pOp4:3xGFP-NLS

    Plasmid
    #116005
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
    Use
    Tags
    linker-NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    116005-plasmid-map-sequence-id-227514

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  15. pBItetDT480GFP

    Plasmid
    #96905
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    96905-plasmid-map-sequence-id-306112

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  16. pBItetDMPKSGFP

    Plasmid
    #96903
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96903-plasmid-map-sequence-id-193288

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  20. pPKm-248

    Plasmid
    #90504
    Purpose
    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    90504-plasmid-map-sequence-id-204704

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Showing: 3641 - 3660 of 4886 results