We narrowed to 9,187 results for: Pol;

1. pLNCX1 HA Akt1

   Plasmid

   #15990

   Depositor

   Morris Birnbaum

   Article

   Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pBAD_Ub11TCA

   Plasmid

   #174517

   Purpose

   Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   Ubiquitin
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Recoded with TCA replacing codon 11 of wt ubiquit…

   Promoter

   araBAD

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 GFP RagB Q99L

   Plasmid

   #112749

   Purpose

   express GFP-RagB (Q99L mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   GFP-RagB (Q99L) (RRAGB Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q99L

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiV_Neo_Sik3_T142Q

   Plasmid

   #119973

   Purpose

   Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Sik3 (Sik3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   change Threonine 142 to Glutamine

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6P1-Ataxin3Q22

   Plasmid

   #22117

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-dCas13b-ADAR2DD

   Plasmid

   #190913

   Purpose

   Addgene #103871 with 3x FLAG on N-terminal of dCas13

   Depositor

   Timothy Jarome

   Article

   Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

   Insert

   3X FLAG
   

   Use

   CRISPR

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF11-SFPQ(276-598)

   Plasmid

   #135437

   Purpose

   Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   Splicing Factor Proline And Glutamine Rich (SFPQ Human)
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 BATF3

   Plasmid

   #67050

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BATF3 (BATF3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

    Plasmid

    #221080

    Purpose

    SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3XFlag-Mettl4

    Plasmid

    #86665

    Purpose

    Expresses murine Mettl4 containing N-terminal 3XFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Mettl4 (Mettl4 Mouse)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYLL204

    Plasmid

    #53455

    Purpose

    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

    Depositor

    Leroy Liu, Lisa Lyu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y814F

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-H2B-mClover3

    Plasmid

    #171489

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H2B (H2BC11 Human)
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIS1 DICER1 long UTR

    Plasmid

    #21649

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 long UTR (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNSSCPG

    Plasmid

    #183187

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xFlag-Alkbh4

    Plasmid

    #105240

    Purpose

    Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Alkbh4 (Alkbh4 Mouse)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 TFAM

    Plasmid

    #67064

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAM (TFAM Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    S115C

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1(+)_UGControl

    Plasmid

    #133875

    Purpose

    Expresses UGControl in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Tags

    flexible linker - mEGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA1 Flag ATXN1L

    Plasmid

    #33242

    Depositor

    Huda Zoghbi

    Article

    Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

    Insert

    ATXN1L (ATXN1L Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

24. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

    Plasmid

    #172226

    Purpose

    Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Piggybac transposon

    Tags

    3XFLAG, DD-domain, GS-linker, and TagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBa.GFP-AP1/beta1

    Plasmid

    #218802

    Purpose

    Mammalian expression o fhuman AP1 b1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    AP1 b1 (AP1B1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Chicken Beta actin

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. -227 hTF (mutant NF-kB)

    Plasmid

    #15443

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    NF-kappaB binding domain mutated

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

28. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. psicheck-2-Casp8

    Plasmid

    #48172

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of CASP8 (CASP8 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only