19,635 results

1. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TBK1

   Plasmid

   #23851

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSII-prEF1a-mCherry-3xNLS

   Plasmid

   #125262

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   mCherry-3xNLS
   

   Use

   Lentiviral

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Peredox-mCherry

   Plasmid

   #32383

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. HTR2A-Tango

   Plasmid

   #66409

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-All-in-one-hCascade

   Plasmid

   #134919

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-IRES Hyg SMAD4

   Plasmid

   #107128

   Purpose

   Constitutive Flag-tagged SMAD4 expression in Mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SuperNova Green/ pRSETB

   Plasmid

   #124839

   Purpose

   bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

   Insert

   SuperNova Green
   

   Tags

   No tag

   Expression

   Bacterial

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140220

    Purpose

    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1
    

    Use

    CRISPR

    Tags

    miniVPR-mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. RAR-TRE-mStrawberry reporter

    Plasmid

    #158683

    Purpose

    This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tag5Amyc-GSK3b WT

    Plasmid

    #16260

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPLICS PM-ER Short P2A

    Plasmid

    #164112

    Purpose

    Detect the short Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MSCV LTR promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-VSVG

    Plasmid

    #1733

    Purpose

    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

    Depositor

    Garry Nolan

    Insert

    VSV-G Envelope
    

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-RhoA

    Plasmid

    #23224

    Depositor

    Channing Der

    Article

    Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

    Insert

    RhoA (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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