We narrowed to 165,347 results for: addgene

1. THI VP64 pGAP T-

   Plasmid

   #161487

   Purpose

   Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Non targeting control NTC)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AQTFAATHNPWASQA_epitope

   Plasmid

   #165019

   Purpose

   Expression of CMV UL86 (1222-1236) fused to CD74

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV UL86 (1222-1236)
   

   Use

   Lentiviral

   Tags

   CD74 at N-term; cathepsin S cleavage site (GRWHTV…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-GFP RanSen

   Plasmid

   #184705

   Purpose

   Lentiviral transfer plasmid for expression of GFP RanSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP RanSen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2363

   Plasmid

   #36776

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   EZH2-TALEN_Right (Ezh2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLpC-L-IFP-F[1] (mammalian)

   Plasmid

   #52901

   Purpose

   Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[1]
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Unknown

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pLpC-L-IFP-F[2] (mammalian)

   Plasmid

   #52902

   Purpose

   Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Unknown

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pFA0055

   Plasmid

   #131774

   Purpose

   Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer CASS5a guide (gCASS5a) and 5' sgRNA
   

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC_N_RAB5A

   Plasmid

   #111692

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   RAB5A (RAB5A Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2403

   Plasmid

   #36806

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   ATM-TALEN_Right (Atm Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pMB1-D1-TelR

    Plasmid

    #204983

    Purpose

    Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFGC5941-PacI-ath-STTM843

    Plasmid

    #84169

    Purpose

    target miRNA843 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM843
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC5941-PacI-ath-STTM845

    Plasmid

    #84170

    Purpose

    target miRNA845 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM845
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE hygro rat IRS-3

    Plasmid

    #11450

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-3 (Irs3 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pICH-chl-roGFP2-GPXC71S

    Plasmid

    #191703

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-redox(ro)GFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PAXBP1_pCSdest

    Plasmid

    #53834

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PAXBP1 (PAXBP1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pRRG227-Wnt1

    Plasmid

    #99073

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Wnt1 (also known as WntP-1) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL2236

    Plasmid

    #36687

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Left (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. B108-Otx2-IRES-Cre

    Plasmid

    #73993

    Purpose

    B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFGC5941-PacI-ath-STTM173

    Plasmid

    #84101

    Purpose

    target miRNA173 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM173
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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