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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1018

Plasmid

#108975

Purpose

shuttle vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1049

Plasmid

#108992

Purpose

Inducible expression vector (dox-on), C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-E

Plasmid

#59796

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele E

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele E

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAJ09

Plasmid

#14913

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis G protein alpha subunit promoter

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-A

Plasmid

#59792

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele A

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele A

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pPI457

Plasmid

#113200

Purpose

shuttle vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, c-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI418

Plasmid

#113199

Purpose

shuttle vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, n-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMM725

Plasmid

#68895

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMM750

Plasmid

#68899

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149S/F366A

Plasmid

#100165

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149S, F366A

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-C

Plasmid

#59794

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele C

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele C

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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