Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,181 results for: nes

Clear keywords
Showing: 3721 - 3740 of 16181 results

  3. pGES401

    Plasmid
    #190198
    Purpose
    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection
    Depositor
    Yuefeng Guan
    Publication
    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190198-plasmid-map-sequence-id-371936

    Enlarge

  7. px458 EQR

    Plasmid
    #101731
    Purpose
    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 EQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101731-plasmid-map-sequence-id-196590

    Enlarge

  9. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

    Enlarge

  11. pmCherry_sgRNA-ver2

    Plasmid
    #126776
    Purpose
    gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA (for SpCas9)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126776-plasmid-map-sequence-id-412379

    Enlarge

  12. p458 VRER

    Plasmid
    #101728
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101728-plasmid-map-sequence-id-196591

    Enlarge

  13. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Publication
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100596-plasmid-map-sequence-id-207339

    Enlarge

  15. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Publication
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

    Enlarge

  17. mPlum-Peroxisomes-2

    Plasmid
    #54509
    Purpose
    Localization: Peroxisomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mPlum-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 3721 - 3740 of 16181 results