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pGP-CMV-NES-jRCaMP1b

Plasmid

#63136

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1b

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-PE2-C

Plasmid

#164908

Purpose

Expresses the C-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

PE2-C

Use

AAV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 WT B-Myb

Plasmid

#25965

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (MYBL2 Human)

Expression

Mammalian

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-lucOS

Plasmid

#129776

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-MP2

Plasmid

#36097

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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SV40 small + Large T in pENTR1A (w611-7)

Plasmid

#22297

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Entry vector

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-myc3-HDM2

Plasmid

#20935

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Mdm2 p53 binding protein homolog (MDM2 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (1B)

Plasmid

#29653

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Sox2

Plasmid

#101761

Purpose

Sox2 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SRY-box 2 promoter (SOX2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Beta1-N-18

Plasmid

#55064

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Expression

Mammalian

Promoter

CAG promotor

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha/TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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