We narrowed to 165,385 results for: addgene

1. THI VP64 pGAP T1

   Plasmid

   #161488

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T1 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAN1_pCSdest

   Plasmid

   #53883

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   RCAN1 (RCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. H2B-CoilE-mEm

   Plasmid

   #181992

   Purpose

   CoilE genetically encoded tag on H2B-mEmerald, to be used with CoilR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   H2B-CoilE-mEm
   

   Tags

   mEmerald

   Expression

   Mammalian

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRG199-bmp4

   Plasmid

   #99076

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Bmp4 (also known as bmp) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_C_TR61B

   Plasmid

   #111679

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TR61B (TRMT61B Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. myc-Rapr-FAK-YM

   Plasmid

   #25927

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-FAK (Ptk2 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   This myc-RapR-FAK-YM plasmid contains FAK mutatio…

   Available Since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLKO.1 shMTH1-1

   Plasmid

   #21297

   Depositor

   Bob Weinberg

   Article

   Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   shMTH1-1 (NUDT1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSLIK 3XFLAG-MKK4-EE-NLS hygro

   Plasmid

   #87779

   Purpose

   Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

10. N6AMT1_pCSdest

    Plasmid

    #53843

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    N6AMT1 (N6AMT1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pFA6a-kanMX6-Purg1-GFP

    Plasmid

    #39353

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP and kanMX6 module - reverse orientation

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

12. sh-RNA hULK2

    Plasmid

    #31970

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pFGC5941-PacI-ath-STTM5026

    Plasmid

    #84192

    Purpose

    target miRNA5026 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM5026
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRAPPC10_pCSdest

    Plasmid

    #53937

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TRAPPC10 (TRAPPC10 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. SIM2_pCSdest

    Plasmid

    #53790

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    SIM2 (SIM2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. VV221: sdChR(C138S)-TS-eGFP-ER in fck

    Plasmid

    #58513

    Purpose

    Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-eGFP-ER
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFGC5941-PacI-ath-STTM398

    Plasmid

    #84124

    Purpose

    target miRNA398 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM398
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. TAL2237

    Plasmid

    #36688

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Right (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFGC5941-PacI-ath-STTM395

    Plasmid

    #84121

    Purpose

    target miRNA395 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM395
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAL2255

    Plasmid

    #36698

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCC-TALEN_Right (Fancc Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only