We narrowed to 5,690 results for: org

1. miniTol2 x EF1a_EKAREN5 + PGK-puro

   Plasmid

   #167817

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

   Plasmid

   #207355

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV x EF1a_EKAREN4-ires-blast

   Plasmid

   #167829

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-PGK-LAMP1-EYFP

   Plasmid

   #164215

   Purpose

   pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   EYFP

   Promoter

   PGK

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-KozATG-dL5-2XG4S-actin

   Plasmid

   #73262

   Purpose

   Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

   Insert

   KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
   

   Tags

   The FAP and actin are fused with 2 copies of a G4…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

   Plasmid

   #180313

   Purpose

   bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Tags

   His6-TEV and sfCherry2

   Expression

   Bacterial

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-HOPX

   Plasmid

   #192923

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOPX (HOPX Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB CAG SMARCA4 T910M-Venus

   Plasmid

   #153951

   Purpose

   piggyBac transposon vector with CAG promoter expressing SMARCA4 (T910M mutant)-Venus fusion protein

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T910M

   Promoter

   CAG

   Available Since

   Aug. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniTol2 x EF1a_EKAREN5 + PGK-blast

   Plasmid

   #167818

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-KLF6

    Plasmid

    #192909

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF6 (KLF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN-L1-K2-L2

    Plasmid

    #80684

    Purpose

    Gateway Entry vector containing a temperature-sensitive mouse DHFR

    Depositor

    Nico Dissmeyer

    Article

    Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

    Inserts

    DHFR (Dhfr Mouse)
    DHFR (Dhfr Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    mouse DHFR with N-terminal Ubiquitin

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

    Plasmid

    #158542

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion, F188D, W2…

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET.SUMO eIF4G1 1-200

    Plasmid

    #158791

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (codon optimized)
    

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MIGR1-Cdc42-1

    Plasmid

    #179517

    Purpose

    Retroviral expression of Cdc42 splice variant 1

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 1 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_SEPT2 NCmut-msfGFP

    Plasmid

    #180320

    Purpose

    mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CuO-V5 CASC3 siRes 110-480 WT

    Plasmid

    #158543

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 110-480, C-terminal deletion

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-R536C-WLS-HA-IRES-GFP

    Plasmid

    #178065

    Purpose

    Expression of WLS-HA protein (R536C mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    R536C

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti HsATP10B WT

    Plasmid

    #171770

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT

    Depositor

    Veerle Baekelandt

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Lentiviral

    Mutation

    D433N

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge