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176,460 results

Showing: 3761 - 3780 of 176460 results

  1. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  3. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139996-plasmid-map-sequence-id-275405

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  5. pLV-EF1-Cre-PGK-Puro

    Plasmid
    #108543
    Purpose
    Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    cre recombinase
    Puromycin
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108543-plasmid-map-sequence-id-208672

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  6. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Publication
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    105829-plasmid-map-sequence-id-203267

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  12. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid
    #67977
    Purpose
    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers
    Depositor
    Kosuke Yusa
    Publication
    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d
    Insert
    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    Use
    Lentiviral
    Mutation
    Deleted BbsI site within WPRE
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    67977-plasmid-map-sequence-id-118694

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  16. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Publication
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    63726-plasmid-map-sequence-id-438433

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Showing: 3761 - 3780 of 176460 results