164,629 results

1. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Michael Davidson, Roger Tsien, Nathan Shaner

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV rtTA3 Hygro (w785-1)

   Plasmid

   #26730

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

   Plasmid

   #139994

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-flex-GFP-4x6T

   Plasmid

   #196418

   Purpose

   Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-NpHR-EYFP

   Plasmid

   #171621

   Purpose

   TRE-NpHR-EYFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-NpHR-EYFP
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi 41BBL-CD86 mIL-15

   Plasmid

   #183245

   Purpose

   Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. phCMV-coGALVenv-C4070A

   Plasmid

   #172217

   Purpose

   Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

   Depositor

   Boris Fehse

   Article

   Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

   Insert

   Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Carries C-terminus of amphotropic MLV strain 4070…

   Promoter

   CMV immediate early promoter

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdx-CMV-copGFP

    Plasmid

    #73346

    Purpose

    Express copGFP under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-copGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hsgk1

    Plasmid

    #83432

    Purpose

    full length human sgk1 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    human Sgk1 (SGK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-sh-mSOX9-5

    Plasmid

    #40646

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-5 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IB-HaloTag7-mGFP-KDEL

    Plasmid

    #184904

    Purpose

    Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    KDEL
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-iGlucoSnFR-TS

    Plasmid

    #127952

    Purpose

    Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

    Depositor

    Gary Yellen

    Article

    Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

    Insert

    iGlucoSnFR-TS
    

    Use

    AAV

    Tags

    His6 and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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