Skip to main content
Addgene
Showing: 721 - 760 of 1734 results

  1. pME_G_0_4_001_CDS_Placeholder

    Plasmid
    #235892
    Purpose
    Encodes a sfGFP cassette in the CDS position used for placeholder cloning
    Depositor
    Tobias Erb
    Insert
    CDS placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235892-plasmid-map-sequence-id-471478

    Enlarge

  2. pME_G_0_2_001_Promoter_placeholder

    Plasmid
    #235808
    Purpose
    Encodes a sfGFP cassette in the promoter position used for placeholder cloning
    Depositor
    Tobias Erb
    Insert
    Promoter placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235808-plasmid-map-sequence-id-471433

    Enlarge

  3. pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

    Plasmid
    #235970
    Purpose
    Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly
    Depositor
    Tobias Erb
    Insert
    Amp/ColEI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    235970-plasmid-map-sequence-id-469960

    Enlarge

  11. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

    Enlarge

  12. pEXP5-NT/pLux LuxI

    Plasmid
    #193627
    Purpose
    Inducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Lux promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193627-plasmid-map-sequence-id-382190

    Enlarge

  36. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

    Enlarge

  40. pDOC-K-glmS-GFPfor

    Plasmid
    #158059
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    acpP
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158059-plasmid-map-sequence-id-309439

    Enlarge

Showing: 721 - 760 of 1734 results