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pSPgRNA_BLK_P3

Plasmid

#124457

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P4

Plasmid

#124458

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P4_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P5

Plasmid

#124459

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P5_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-1

Plasmid

#124474

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-1_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-2

Plasmid

#124475

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-2_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-3

Plasmid

#124476

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-4

Plasmid

#124477

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-4_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E3-3

Plasmid

#124484

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E3-3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 CortW22A mCherry

Plasmid

#187268

Purpose

Lenticiral expression of mCherry-Cortactin W22A

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mcherry

Mutation

W22A

Promoter

5' LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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BMP10 N67Q N131Q D4G (S2-2)

Plasmid

#184622

Purpose

for transfection into S2 cells and expression of BMP10 protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

BMP10 (BMP10 Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Mutation

N67Q, N131Q, ARIRR(312-316) replaced with G

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

Plasmid

#175586

Purpose

in vitro transcription of mut RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz mut 2598-4066

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

add arginines

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

remove all K, most D

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_04

Plasmid

#158105

Purpose

CaV1.3 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1D (uc003dgu.5)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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