We narrowed to 9,202 results for: sacs

1. pJO629

   Plasmid

   #104342

   Purpose

   Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

   Insert

   pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. UAS:Zebrabow-V

   Plasmid

   #118218

   Purpose

   For broad or variegated gal4 inducible expression of Brainbow in zebrafish

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK255

   Plasmid

   #171155

   Purpose

   Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   I-SceI_sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Plasmid

   #58440

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA-Gent-EM7-Erv1p

   Plasmid

   #202484

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sc eRF3∆165N (WT)

   Plasmid

   #175418

   Purpose

   Encodes His-MBP-TEV-WT eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   ∆165N

   Promoter

   T7

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMM4

   Plasmid

   #170359

   Purpose

   Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

   Depositor

   Marvin Wickens, Hugo Cesar Medina-Munoz

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PCYC1_SEC61_mCherry_LEU2_TCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PCYC1

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-Flag-mEPLIN alpha

   Plasmid

   #40955

   Depositor

   Scott Maul, Elizabeth Luna

   Article

   Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

   Insert

   EPLIN alpha (Lima1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. S3_001_026_MV2

    Plasmid

    #134422

    Purpose

    pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Endoplasmic reticulum-targeted optodPLD

    Plasmid

    #140059

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CIBN, CRY2, P2A, Sac1(TM), and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-Vps4

    Plasmid

    #87733

    Purpose

    Bacterial expression of full-length S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-LifeAct-Venus

    Plasmid

    #87613

    Purpose

    Actin-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-Venus
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CuGFP-ATG8(416)

    Plasmid

    #49423

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

    Depositor

    Daniel Klionsky

    Article

    Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Plas-gRNA-Z3EV

    Plasmid

    #157659

    Purpose

    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA NatMX Z3EV
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. yHS1

    Plasmid

    #159150

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDS277

    Plasmid

    #34973

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V529N

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK332

    Plasmid

    #59573

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIplac211-VPS8-HaloTagx2

    Plasmid

    #177113

    Purpose

    Expresses Vps8-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    VPS8 (VPS8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-H3.3-YFP

    Plasmid

    #8693

    Depositor

    Kami Ahmad

    Article

    Janicki et al Cell 2004 Mar 5;116(5):683-98.

    Insert

    H3.3A (His3.3A Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSV(X) C/EBPdelta

    Plasmid

    #13394

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPdelta (Cebpd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pFA6a-kanMX6-Purg1

    Plasmid

    #39349

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    kanMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNC1011

    Plasmid

    #42898

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    3 tandem copies of GFP*
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    changed Phenylalanine 64 to Leucine, Serine 65 t…

    Promoter

    None

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. raACR-pmCerulean3-N1

    Plasmid

    #204959

    Purpose

    Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge