164,577 results

1. pEGFP-C1-hAtg2A

   Plasmid

   #36456

   Depositor

   Noboru Mizushima

   Article

   Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

   Insert

   autophagy-related protein 2 homolog A (ATG2A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed Proline 656 to Arginine

   Promoter

   CMV

   Available Since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. DUSP6

   Plasmid

   #27975

   Depositor

   Igor Astsaturov

   Article

   Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

   Insert

   DUSP6 (DUSP6 Human)
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pflp-18::NGL-1::spGFP11

   Plasmid

   #65828

   Purpose

   For trans-synaptic labeling

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Use

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   N142S

   Promoter

   Pflp-18

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. moxBFP

   Plasmid

   #68064

   Purpose

   C-terminal fusion to oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   moxBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRG (Lenti_sgRNA_EFS_GFP)

   Plasmid

   #65656

   Purpose

   Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter-driven sgRNA expression and EFS promo…

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. DHFR-SpCas9-DHFR

   Plasmid

   #85447

   Purpose

   Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-SpCas9-DHFR
   

   Use

   AAV

   Tags

   Destabilized domain (C-terminus version) of E.col…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_NOTCH2_WT

   Plasmid

   #82292

   Purpose

   Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   C946Y

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

    Plasmid

    #62897

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS6-YFP-SMASh(TI)

    Plasmid

    #111501

    Purpose

    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    YFP-SMASh(TI)
    

    Use

    Tags

    HA epitope tag and SMASh tag

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, S122R, a…

    Promoter

    CMV

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-GFP SNAP25

    Plasmid

    #170872

    Purpose

    Mammalian expression of His-GFP SNAP25.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    SNAP 25 (Snap25 Rat)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ubi:Zebrabow

    Plasmid

    #118219

    Purpose

    For ubiquitous expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCMV_COCVmut_G

    Plasmid

    #207262

    Purpose

    Mammalian expression of a mutant Cocal virus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    COCVmut_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47A/Y209A/R354A; mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-eGFP-beta 2 CP

    Plasmid

    #13298

    Depositor

    John Cooper

    Article

    Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

    Insert

    capping protein beta 2 subunit (Capzb Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. T5-pAc5.1 EGFP-EcoRV

    Plasmid

    #206481

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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