We narrowed to 9,187 results for: Pol;

1. pYES2/103Q

   Plasmid

   #1385

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (103Q) (HTT Human)
   

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGB_YFP-MiniTurboID

   Plasmid

   #222433

   Purpose

   Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmRFP-N1 human cofilin S3E

   Plasmid

   #50858

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGM227

   Plasmid

   #220182

   Purpose

   Expresses exogenous wild-type TOMM70A

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TOMM70A (TOMM70 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2022

   Plasmid

   #49129

   Purpose

   AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-PRS2/3:Con-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   DBH

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCA13-pHR-SFFV-HaloTag-FBL

   Plasmid

   #199444

   Purpose

   Expression of HaloTag-FBL in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   FBL (FBL Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C-p50

    Plasmid

    #180238

    Purpose

    Expresses p50 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guo et al Proc Natl Acad Sci U S A. 2016 Sep 6;113

    Insert

    p50 (DCTN2 Human)
    

    Tags

    pmCherry-C1

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Tags

    Cerulean

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMT-Mef2c-tPT2A-GFP

    Plasmid

    #111771

    Purpose

    Bicistronic construct: Co-express two genes by 2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Insert

    Mef2c-tPT2A-GFP (Mef2c Mouse)
    

    Use

    Cloning intermediate

    Tags

    GFP

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL Blast Snail ER

    Plasmid

    #18798

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    snail (SNAI1 Human)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV N-term HA-FLAG puro BRD7

    Plasmid

    #41934

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    HA-Flag

    Expression

    Mammalian

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pQFlag-USP7 CS puroR

    Plasmid

    #46752

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-H1-shScramble-hsynapsin-EGFP

    Plasmid

    #220795

    Purpose

    control shRNA plasmid with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG-Crx-IRES-GFPd2

    Plasmid

    #73997

    Purpose

    Plasmid expressing Crx-IRES- GFPd2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Crx-IRES-GFPd2 (Crx Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV6-AN-HA_WT RAD23A

    Plasmid

    #201458

    Purpose

    Expresses human RAD23A with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1 Dynamin1 T292A L293A P294A

    Plasmid

    #112104

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T292A L293A P294A

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-N1 Dynamin1 L330R Q334R L702R

    Plasmid

    #112105

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L330R Q334R L702R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-N1 Dynamin1 D406R M407R T488W

    Plasmid

    #112111

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406R M407R T488W

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWZL Blast Twist ER

    Plasmid

    #18799

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGEX4T3-GST-NQO1

    Plasmid

    #177140

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDFDuet-nsp7-nsp8

    Plasmid

    #159092

    Purpose

    Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

    Depositor

    Seth Darst

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Inserts

    NSP7
    NSP8
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF myc Gal cyclin T1 (1-726) (P#1026)

    Plasmid

    #14626

    Depositor

    Matija Peterlin

    Article

    Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

    Insert

    cyclin T1 (CCNT1 Human)
    

    Tags

    Gal and myc

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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