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pMaCTag-P04

Plasmid

#120015

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Desmocollin2_tail-GST

Plasmid

#36992

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsc2 (DSC2 Human)

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of Dsc2 (nt 2,603–3,158)

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
TAL2314

Plasmid

#36740

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCF-TALEN_Left (Fancf Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pFP.R271

Plasmid

#138220

Purpose

Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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WDR34

Plasmid

#132526

Purpose

Human WDR34, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR34 (WDR34 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TCTEX1D2

Plasmid

#132529

Purpose

Human TCTEC-1D2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

TXCTEX1D2 (TCTEX1D2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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WDR60

Plasmid

#132525

Purpose

Human WDR60, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR60 (WDR60 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT L79E

Plasmid

#13640

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Leu 79 to Glu

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
P16ΔS:(MCS)-3xHA-S11

Plasmid

#108185

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2416

Plasmid

#36817

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCA-TALEN_Left (Fanca Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-DEL

Plasmid

#103035

Purpose

[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by DEL domian

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-DEL

Use

T3 in vitro transcription for expression in mamma…

Tags

DEL and HA

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2275

Plasmid

#36712

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Right (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2400

Plasmid

#36803

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Left (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2296

Plasmid

#36729

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BAX-TALEN_Left (Bax Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2297

Plasmid

#36730

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BAX-TALEN_Right (BAX Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2328

Plasmid

#36754

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MLH1-TALEN_Left (Mlh1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2329

Plasmid

#36755

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MLH1-TALEN_Right (Mlh1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2413

Plasmid

#36814

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BCL6-TALEN_Right (Bcl6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
mCherry-SAC1C389S-FKBP

Plasmid

#108130

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S:FKBP(3-108) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2361

Plasmid

#36774

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX8-TALEN_Right (Cbx8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2414

Plasmid

#36815

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT1-TALEN_Left (Ext1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_PKA-CαN15-mVenus

Plasmid

#168486

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2412

Plasmid

#36813

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BCL6-TALEN_Left (Bcl6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2401

Plasmid

#36804

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Right (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

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