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mEmerald-MCM3-C-19

Plasmid

#54165

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Anti-Olig1 [N149/25.1R]

Plasmid

#114540

Purpose

Mammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Krox20 promoter pGL3-TATA

Plasmid

#21260

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20 promoter (Egr2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

Plasmid

#156430

Purpose

Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

CTCF, rtta3G, TetO3G (Ctcf Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

Cterm_Nterm_switched

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3

Plasmid

#52371

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

Ub

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

Plasmid

#159912

Purpose

Mutagenesis of Kcnd3

Depositor

Larry Zweifel

Insert

Kcnd3 (Kcnd3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgMettl3

Plasmid

#196263

Purpose

Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 sgRNA (Mettl3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kir2.1 K+ channel [N112B/22R]

Plasmid

#220400

Purpose

Mammalian Expression Plasmid of anti-Kir2.1 K+ channel (Mouse) IgG2a R-mAb. Derived from hybridoma N112B/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kir2.1 K+ channel (Mus musculus) recombinant mouse monoclonal antibody. (Kcnj2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112/16]

Plasmid

#206769

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/29]

Plasmid

#206770

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112B/29 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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WZLneo-C/EBPα

Plasmid

#34567

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

C/EBPα (Cebpa Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mdnTcf4Ben (CT#1130)

Plasmid

#14020

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Mutation

Dominant negative Tcf4B, engrailed fusion

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pRL c-Myc 3'UTR

Plasmid

#14806

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

c-Myc 3'UTR (Myc Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
MEIS1A-pVP16

Plasmid

#21016

Depositor

Corey Largman

Insert

MEIS1A (Meis1 Mouse)

Tags

VP16-AD

Expression

Mammalian

Mutation

VP16 activation domain fused to N-terminal

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pM5MF529

Plasmid

#17686

Depositor

David Shalloway

Article

Lin et al Oncogene. 1995 Jan 19. 10(2):401-5.

Insert

c-src Y529F (Src Mouse)

Expression

Mammalian

Mutation

Tyr 529 changed to Phe .

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Synthetic, Aequorea victoria, Mouse)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC Flag-mRAP1

Plasmid

#73135

Purpose

Expression of Flag-tagged mRAP1

Depositor

Titia de Lange, Agnel Sfeir

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR223 R2pH-LAMP1-3xFLAG

Plasmid

#157941

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG, mCherry, and pHluorin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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