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pFB-His-SKP1

Plasmid

#228368

Purpose

express human SKP1 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

SKP1 (SKP1 Human)

Tags

His

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1

Plasmid

#232273

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FBXO22

Plasmid

#232271

Purpose

Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBiFC-SS-VC155-IRE1-dLD

Plasmid

#217751

Purpose

Expresses the VC fragment of BiFC-IRE1-dLD

Depositor

Christina Chan

Article

Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

Insert

VC155-IRE1-dLD (ERN1 Human)

Tags

Venus (155-238, A206K)

Expression

Mammalian

Mutation

deleted amino acids 29-408

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBiFC-SS-VN155(I152L)-IRE1-dLD

Plasmid

#217752

Purpose

Expresses the VN fragment of BiFC-IRE1-dLD

Depositor

Christina Chan

Article

Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

Insert

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)

Tags

Venus (1-154, I152L)

Expression

Mammalian

Mutation

deleted amino acids 29-408

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5B-SNAPf-HisTag-FLAG

Plasmid

#200799

Purpose

Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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psicheck2-PHLPP2mut

Plasmid

#203606

Purpose

Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR were m…

Promoter

SV40

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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B-HiFi SpCas9

Plasmid

#207362

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2281

Plasmid

#142929

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYBBP1A (MYBBP1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TMIGD2-COMP5AP-AviTag-9xHis

Plasmid

#157080

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TMIGD2 (TMIGD2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3155

Plasmid

#144631

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HLX (HLX Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD4

Plasmid

#121108

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N601A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N601A)

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD5

Plasmid

#121109

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N739A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N739A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD1

Plasmid

#121105

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (Y127A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (Y127A)

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD3

Plasmid

#121107

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N463A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N463A)

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD2

Plasmid

#121106

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N263A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N263A)

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG_PSD95.FingR-eGFP-CCR5TC

Plasmid

#46295

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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